Affiliation:
1. College of Chemistry and Materials Science Hebei University Key Laboratory of Analytical Science and Technology of Hebei Province Baoding 071002 People's Republic of China
2. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong School of Chemistry and Chemical Engineering University of Jinan Jinan 250022 People's Republic of China
3. Difficult and Severe Liver Disease Center Baoding People's Hospital Baoding 071030 People's Republic of China
Abstract
AbstractIn this work, a highly sensitive and selective method for detecting folic acid (FA) was developed using D‐penicillamine (DPA) stabilized Ag/Cu alloy nanoclusters (DPA@Ag/Cu NCs). The yellow emission of DPA@Ag/Cu NCs was found to be quenched upon the addition of FA to the system. The fluorescence intensity quenching value demonstrated a linear relationship with FA concentrations ranging from 0.01 to 1200 μM, with a limit of detection (LOD) of 5.3 nM. Furthermore, the detection mechanism was investigated through various characterization analyses, including high resolution transmission electron microscopy, fluorescence spectra, ultraviolet‐visible absorption spectra, and fluorescence lifetime. The results indicated that the fluorescence quenching induced by FA was a result of electron transfer from FA to the ligands of DPA@Ag/Cu NCs. The selectivity of the FA sensor was also evaluated, showing that common amino acids and inorganic ions had minimal impact on the detection of FA. Moreover, the standard addition method was successfully applied to detect FA in human serum, chewable tablets and FA tablets with promising results. The use of DPA@Ag/Cu NCs demonstrates significant potential for detecting FA in complex biological samples.
Funder
Natural Science Foundation of Shandong Province