Affiliation:
1. Schulich Faculty of Chemistry Technion-Israel Institute of Technology Haifa 3200008 Israel
Abstract
AbstractWe designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi‐synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel–Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post‐translationally modified proteins in live cells.
Subject
Organic Chemistry,Molecular Biology,Molecular Medicine,Biochemistry