Affiliation:
1. Department of Physical Chemistry and Technology of Polymers, Faculty of Chemistry Silesian University of Technology Gliwice Poland
2. Department of Systems Biology and Engineering, Faculty of Automatic Control, Electronics and Computer Science Silesian University of Technology Gliwice Poland
3. Biotechnology Centre Silesian University of Technology Gliwice Poland
Abstract
AbstractIn vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, that is, p‐aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried out. These systems were tested against normal: human bronchial epithelial cells (BEAS‐2B), and cancers: adenocarcinoma human alveolar basal epithelial cells (A549), and human non‐small cell lung carcinoma cell line (H1299). Cells viability, after linear copolymer LC and their conjugates addition for 72 h, was measured at concentration range of 3.125–100 μg/mL. The MTT test allowed the designation of IC50 index, which was higher for BEAS‐2B, and significantly lower in the case of cancer cell lines. The cytometric analyzes, that is, Annexin‐V FITC apoptosis assay and cell cycle analysis as well as gene expression measurements for interleukins IL6 and IL8 were carried out, and showed pro‐inflammatory activity of tested compounds toward cancer cells, while it was not observed against normal cell line.
Subject
Biomedical Engineering,Biomaterials
Cited by
2 articles.
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