Affiliation:
1. College of Biochemical Engineering, Beijing Key Laboratory of Bioactive Substances and Functional Food Beijing Union University Beijing China
2. College of Food Science and Engineering Northwest A&F University Xianyang China
Abstract
AbstractFood‐sourced proteins have been shown to have ameliorative effects on ulcerative colitis (UC), and studies have identified potential anti‐inflammatory activity of silkworm pupa proteins. Here, we identified and screened two novel protein‐derived peptides, PDLGLF (PD‐6) and GTEGGFPF (GT‐8), from Bombyx mori using UPLC–MS/MS and bioinformatics analysis. These peptides can serve as effective experimental drugs for colitis by modulating the gut microbiota. Treatment with peptides PD‐6 and GT‐8 inhibited colitis inflammation in UC mice, improved oxidative stress levels, and maintained intestinal epithelial barrier function. Peptides PD‐6 and GT‐8 also altered the diversity and composition of the gut microbiota in UC mice and increased the concentration of short‐chain fatty acids. In conclusion, these findings reveal the potential of the naturally derived bioactive peptides PD‐6 and GT‐8 in controlling intestinal inflammation and underscore the potential of PD‐6 and GT‐8 as promising candidates for the prevention and improvement of UC‐related inflammatory diseases.