Affiliation:
1. Institute of Pesticide Science, College of Plant Protection Northwest A&F University Yangling Shaanxi China
2. Key Laboratory for Botanical Pesticide R&D of Shaanxi Province Northwest A&F University Yangling Shaanxi China
3. Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education Northwest A&F Univeristy Yangling Shaanxi China
Abstract
AbstractBACKGROUNDPeriplocoside P (PSP) is a major component of Periploca sepium Bunge known for its potent insecticidal activity. V‐Type adenosine triphosphatase (V‐ATPase), which is widely distributed in the cytoplasmic membranes and organelles of eukaryotic cells, plays a crucial role in synaptic excitability conduction. Previous research has shown that PSP targets the apical membrane of goblet cells in the insect midgut. However, the effects of PSP on synaptic transmission at the neuromuscular junction are often overlooked.RESULTSThe bioassay revealed that Drosophila adults with different genetic backgrounds showed varying levels of susceptibility to PSP in the order: parats1 > parats1;DSC1−/− ≈ w1118 > DSC1−/−. Intracellular electrode recording demonstrated that PSP, similar to bafilomycin A1, had an impact on the amplitude of the excitatory junction potential (EJP) and accelerated excitability decay. Furthermore, the alteration in EJP amplitude is concentration‐dependent. Another surprising discovery was that the knockout DSC1 channel showed insensitivity to PSP.CONCLUSIONOur findings confirm that PSP can influence synaptic transmission at the neuromuscular junction of Drosophila larvae by targeting V‐ATPase. These results provide a basis for investigating the mechanism of action of PSP and its potential application in designing novel insecticides. © 2023 Society of Chemical Industry.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Insect Science,Agronomy and Crop Science,General Medicine