Development and validation of an RT-qPCR assay for rapid detection and quantification of hepatitis C virus RNA for routine testing in Moroccan clinical specimens

Author:

El Hadi Hicham12,Benani Abdelouaheb3,Qmichou Zineb1,Lazar Fatiha3,Bakri Youssef2,Ait Benhassou Hassan1,Moumen Abdeladim1ORCID

Affiliation:

1. Division of Biotechnology; Medical Biotechnology Center, Moroccan Foundation for Advanced Science Innovation and Research (MASCIR); Rabat Morocco

2. Biology of Human Pathologies Laboratory, Faculty of Science; Mohamed V University; Rabat Morocco

3. Molecular Biology Laboratory; Pasteur Institute of Morocco; Casablanca Morocco

Funder

Moroccan foundation for advanced science, innovation and research

Publisher

Wiley

Subject

Infectious Diseases,Virology

Reference33 articles.

1. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome;Choo;Science,1989

2. Expression and characterization of recombinant E2 protein of hepatitis C virus by insect cell/baculovirus expression system;Han;J Microbiol Biotechnol,1998

3. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis;Kuo;Science,1989

4. A novel protein to bind HCV core protein: the carboxyl terminus-truncated core120 protein of HCV interacts with E7 antigen of human papilloma virus type 18;So;J Microbiol Biotechnol,2002

5. Outbreak of hepatitis C virus infection in patients with hematologic disorders treated with intravenous immunoglobulins: different prognosis according to the immune status;Rossi;Blood,1997

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