Identification of potential molecular mechanism related to craniofacial dysmorphism caused by FOXI3 deficiency

Author:

Xing Xiao‐Liang12,Zeng Ziqiang12ORCID,Wang Yana1,Pan Bo3,Huang Xueshuang12ORCID

Affiliation:

1. School of Basic Medicine Ningxia Medical University Yinchuan Ningxia China

2. Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine Hunan University of Medicine Changsha China

3. Department of Auricular Reconstruction, Plastic Surgery Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

Abstract

AbstractBackgroundHemifacial macrosomia (HFM, OMIM 164210) is a complex and highly heterogeneous disease. FORKHEAD BOX I3 (FOXI3) is a susceptibility gene for HFM, and mice with loss of function of Foxi3 did exhibit a phenotype similar to craniofacial dysmorphism. However, the specific pathogenesis of HFM caused by FOXI3 deficiency remains unclear till now.MethodIn this study, we first constructed a Foxi3 deficiency (Foxi3−/−) mouse model to verify the craniofacial phenotype of Foxi3−/− mice, and then used RNAseq data for gene differential expression analysis to screen candidate pathogenic genes, and conducted gene expression verification analysis using quantitative real‐time PCR.ResultsBy observing the phenotype of Foxi3−/− mice, we found that craniofacial dysmorphism was present. The results of comprehensive bioinformatics analysis suggested that the craniofacial dysmorphism caused by Foxi3 deficiency may be involved in the PI3K‐Akt signaling pathway. Quantitative real‐time PCR results showed that the expression of PI3K‐Akt signaling pathway‐related gene Akt2 was significantly increased in Foxi3−/− mice.ConclusionThe craniofacial dysmorphism caused by the deficiency of Foxi3 may be related to the expression of Akt2 and PI3K‐Akt signaling pathway. This study laid a foundation for understanding the function of FOXI3 and the pathogenesis and treatment of related craniofacial dysmorphism caused by FOXI3 dysfunction.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Reference51 articles.

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