Affiliation:
1. Faculty of Sciences and Mathematics University of Priština in Kosovska Mitrovica Lole Ribara 29 38220 Kosovska Mitrovica Serbia
2. Vinča Institute of Nuclear Sciences University of Belgrade P.O. Box 522 11000 Belgrade Serbia
3. Faculty of Technology and Metallurgy University of Belgrade Karnegijeva 4 11000 Belgrade Serbia
4. Faculty of Chemistry University of Bialystok Ciołkowskiego 1 K 15-245 Białystok Poland
Abstract
AbstractThe silver and gold L‐methionine capped nanoparticles (Ag and Au @LM NPs) were analyzed as prospective acetylcholinesterase (AChE) inhibitors to test their potential in the treatment of cognitive impairment in depression and Alzheimer's disease. The stability of NPs, and their ability to inhibit AChE were studied by UV‐Vis and FTIR spectrophotometry. At the same time, TEM and SEM measurements, DLS, and zeta potential measurements were employed in the structural characterization of NPs. Nearly spherical, negatively charged Ag and Au @LM NPs, with 17 nm and 31 nm in diameter, respectively, showed moderate inhibitory potential toward AChE in the given frame of investigated concentrations. For both NPs IC50 is not reached. Furthermore, the adsorption of enzyme molecules on the surface of Ag and Au @LM NPs was demonstrated. Hence, our assumption is that inhibition of AChE is caused by blockage of the enzyme‘s active site due to the steric hindrance of NPs.
Funder
Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja