Affiliation:
1. Department of Pathology University of California San Diego La Jolla California
2. Sanford Consortium for Regenerative Medicine University of California San Diego La Jolla California
3. Department of Neurology Northwestern University Chicago Illinois
Abstract
AbstractWe previously established a trophoblast differentiation protocol from primed human pluripotent stem cells (PSC). To induce this lineage, we use a combination of Bone Morphogenetic Protein‐4 (BMP4) and the WNT inhibitor IWP2. This protocol has enabled us to obtain a pure population of trophectoderm (TE)‐like cells that could subsequently be terminally differentiated into syncytiotrophoblasts (STB) and extravillous trophoblasts (EVT). However, the resulting TE‐like cells could only be terminally differentiated to a variable mixture of STB and EVT, with a bias toward the STB lineage. Recently, methods have been developed for derivation and culture of self‐renewing human trophoblast stem cells (TSC) from human embryos and early gestation placental tissues. These primary TSCs were further able to differentiate into either STB or EVT with high efficiency using the lineage specific differentiation protocols. Based partly on these protocols, we have developed methods for establishing self‐renewing TSC‐like cells from PSC, and for efficient lineage‐specific terminal differentiation. Here, we describe in detail the protocols to derive and maintain PSC‐TSC, from both embryonic stem cells (ESC) and patient‐derived induced pluripotent stem cells (iPSC), and their subsequent terminal differentiation to STB and EVT. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Trophoblast Differentiation into TE‐like CellsBasic Protocol 2: Conversion of PSC‐Derived TE‐like Cells to TSCBasic Protocol 3: Passaging PSC‐Derived TSC in iCTB Complete MediumBasic Protocol 4: STB Differentiation from PSC‐derived TSCBasic Protocol 5: EVT Differentiation from PSC‐derived TSCSupport Protocol 1: Geltrex‐coated tissue culture plate preparationSupport Protocol 2: Collagen IV‐coated tissue culture plate preparationSupport Protocol 3: Fibronectin‐coated tissue culture plate preparation
Subject
Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience