Affiliation:
1. Human Immunology Core University of Iowa Iowa City Iowa USA
2. Holden Comprehensive Cancer Center University of Iowa Iowa City Iowa USA
3. Department of Microbiology and Immunology, Carver College of Medicine University of Iowa Iowa City Iowa USA
Abstract
AbstractWe have developed a 31‐color panel to define the steady‐state phenotype of T cells in human peripheral blood (Table 1). The panel presented here was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in this panel were chosen in order to characterize the steady‐state phenotype of T cells and includes markers (CD45RA, CD45RO, CCR7, CD95) to distinguish the main subsets (e.g., naïve, TEM, TCM, TEMRA, TSCM etc.) of CD4, CD8, and γδ T cells. This panel also includes markers for the identification of differentiation status (CD27, CD28), activation/antigen experience status (CD11a, CD49d, CD38, HLA‐DR, CD56, and CD39), co‐inhibitory marker expression (PD‐1, TIM‐3), and CD4 T helper subsets (CXCR3, CXCR5, CCR4, CCR6, Foxp3, CD25, and CD127). This optimized panel provides a broad assessment of the steady‐state phenotype of human T cells.
Funder
National Institutes of Health
Subject
Cell Biology,Histology,Pathology and Forensic Medicine