A Modified Injector and Sample Acquisition Protocol Can Improve Data Quality and Reduce Inter‐Instrument Variability of the Helios Mass Cytometer-Reference-Cited by-同舟云学术

A Modified Injector and Sample Acquisition Protocol Can Improve Data Quality and Reduce Inter‐Instrument Variability of the Helios Mass Cytometer

Published:2019-07-30 Issue:9 Volume:95 Page:1019-1030
ISSN:1552-4922
Container-title:Cytometry Part A
language:en
Short-container-title:Cytometry

Author:

Lee Brian H.¹,Kelly Geoffrey¹,Bradford Shermineh¹,Davila Melanie¹,Guo Xinzheng V.¹,Amir El‐Ad David²,Thrash Emily M.³,Solga Michael D.⁴,Lannigan Joanne⁴,Sellers Brian⁵,Candia Julian⁵,Tsang John⁵,Montgomery Ruth R.⁶,Tamaki Stanley J.⁷,Sigdel Tara K.⁷,Sarwal Minnie M.⁷,Lanier Lewis L.⁸,Tian Yuan⁹,Kim Cheryl¹⁰,Hinz Denise¹⁰,Peters Bjoern⁹¹¹,Sette Alessandro⁹¹¹,Rahman Adeeb H.¹¹²^ORCID

Affiliation:

1. Human Immune Monitoring CenterIcahn School of Medicine at Mt. Sinai New York New York

2. Astrolabe Diagnostics Inc. Fort Lee New Jersey

3. Center for Immuno‐Oncology, Department of Medical OncologyDana‐Farber Cancer Institute Boston Massachusetts

4. Department of Microbiology, Immunology, and Cancer BiologyUniversity of Virginia Charlottesville Virginia

5. Trans‐NIH Center for Human Immunology, Autoimmunity, and InflammationNational Institutes of Health Bethesda Maryland

6. Department of Internal Medicine, School of MedicineYale University New Haven Connecticut

7. Department of SurgeryUniversity of California San Francisco San Francisco California

8. Department of Microbiology and ImmunologyUniversity of California San Francisco San Francisco California

9. Division of Vaccine DiscoveryLa Jolla Institute for Immunology La Jolla California

10. Flow Cytometry Core FacilityLa Jolla Institute for Immunology La Jolla

11. Department of MedicineUniversity of California San Diego La Jolla California

12. Department of Genetics and Genomic SciencesIcahn School of Medicine at Mt. Sinai New York New York

Funder

National Cancer Institute

National Institute of Allergy and Infectious Diseases

NIH Office of the Director

Publisher

Wiley

Subject

Cell Biology,Histology,Pathology and Forensic Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.a.23866

Reference10 articles.

1. Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry

2. Average Overlap Frequency: A simple metric to evaluate staining quality and community identification in high dimensional mass cytometry experiments

3. Optimization of mass cytometry sample cryopreservation after staining

4. Cytobank: Providing an analytics platform for community cytometry data analysis and collaboration;Chen TJ;Curr Top Microbiol Immunol,2014

5. Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

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