Optimization in Detection of Antigen‐Specific T Cells Through Differentially Labeled MHC Multimers-Reference-Cited by-同舟云学术

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Optimization in Detection of Antigen‐Specific T Cells Through Differentially Labeled MHC Multimers

Published:2019-12-06 Issue:9 Volume:97 Page:955-964
ISSN:1552-4922
Container-title:Cytometry Part A
language:en
Short-container-title:Cytometry

Author:

Pedersen Natasja Wulff¹^ORCID,Laske Karoline²³,Maurer Dominik³,Welters Marij⁴,Walter Steffen⁵,Gouttefangeas Cécile²,Hadrup Sine Reker¹

Affiliation:

1. Department of Health Technology Technical University of Denmark Copenhagen Denmark

2. Department of Immunology, Institute of Cell Biology University of Tübingen Tübingen Germany

3. Immatics Biotechnologies GmbH Tübingen Germany

4. Department of Clinical Oncology Leiden University Medical Center Leiden Netherlands

5. Immatics US, Inc Houston Texas 77030 USA

Publisher

Wiley

Subject

Cell Biology,Histology,Pathology and Forensic Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.a.23942

Reference14 articles.

1. Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers

2. Simultaneous detection of many T-cell specificities using combinatorial tetramer staining

3. A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

4. The CIMT-monitoring panel: a two-step approach to harmonize the enumeration of antigen-specific CD8+ T lymphocytes by structural and functional assays

5. Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

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