Determination of background, signal-to-noise, and dynamic range of a flow cytometer: A novel practical method for instrument characterization and standardization

Author:

Giesecke Claudia12,Feher Kristen3,von Volkmann Konrad4,Kirsch Jenny3,Radbruch Andreas1,Kaiser Toralf3

Affiliation:

1. Department of Cell Biology; German Rheumatism Research Center, Leibniz Institute; Berlin Germany

2. Berlin-Brandenburg School for Regenerative Therapies (BSRT); Berlin Germany

3. Department for Cytometry and Cellsorting; German Rheumatism Research Center, Leibniz Institute; Berlin Germany

4. APE Angewandte Physik und Elektronik GmbH; Berlin Germany

Funder

Berlin-Brandenburg School for Regenerative Therapies

European Regional Development Fund (Europäischer Fonds für regionale Entwicklung (EFRE)), Germany

Publisher

Wiley

Subject

Cell Biology,Histology,Pathology and Forensic Medicine

Reference18 articles.

1. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols;Kalina;Leukemia,2012

2. Evaluating fluorescence sensitivity on flow cytometers: An overview;Wood;Cytometry,1998

3. Standardization, Calibration, and Control in Flow Cytometry

4. Noise, sensitivity, and resolution of flow cytometers;Steen;Cytometry,1992

5. Hoffman RA Wood JCS. Characterization of flow cytometer instrument sensitivity Wiley 2001 Curr Protoc Cytom

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