Controllable Preparation of 2D V2O5 Peroxidase‐Mimetic Nanozyme to Develop Portable Paper‐Based Analytical Device for Intelligent Pesticide Assay

Abstract

AbstractGiven severe harmfulness of pesticides, unique characteristics of peroxidase‐mimetic nanozymes, and favorable prospects of paper‐based analytical devices (PADs), it is highly desirable to construct a nanozyme‐based PAD for intelligent analysis of pesticide without enzyme/aptamer/antibody and interference of O2. Herein, 2D nanosheet‐like V2O5 (2D‐VONz) with exclusive peroxidase‐mimetic activity is controllably prepared under the optimal reactants concentration and reaction temperature. Experimental characterizations demonstrate that 2D‐VONz exhibits high affinity and catalytic rate, and catalytic oxidation is dependent on •OH yielded from the decomposition of H2O2 catalyzed by 2D‐VONz, and the catalytic performance is relevant to π–π stacking force‐controlled surface zeta potential of 2D‐VONz changed by substrates, giving a comprehensive understand of the inherent mechanism. Interestingly, 2D‐VONz activity is inhibited by pesticide glyphosate (Gly), and then is exploited to develop a PAD, on which, Gly declines 2D‐VONz activity to prevent it from catalyzing the oxidation of 3,3′,5,5′‐tetramethylbenzidine, contributing to rapid, naked‐eye, and portable analysis of pesticide using a smartphone. The current strategy on preparing exclusive peroxidase‐mimetic 2D nanozyme, investigating catalytic mechanism, developing nanozyme‐based PAD, and achieving direct pesticide sensing will set up new avenues to improve the analytical performance, strengthen the practicability, and broaden the application scope of nanozymes.