Label‐Free and Colorimetric Detection of Influenza A Virus via Receptor‐Mediated Viral Fusion with Plasmonic Vesicles
Author:
Moon Yesol1,
Lee Sojeong1,
Kim Jinyoung1,
Park Geunseon1,
Park Chaewon1,
Lim Jong‐Woo2,
Yeom Minjoo2,
Song Daesub2,
Haam Seungjoo1ORCID
Affiliation:
1. Department of Chemical and Biomolecular Engineering Yonsei University Seoul 03722 Republic of Korea
2. Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seoul 08826 Republic of Korea
Abstract
AbstractThe rapid transmission and numerous re‐emerging human influenza virus variants that spread via the respiratory system have led to severe global damage, emphasizing the need for detection tools that can recognize active and intact virions with infectivity. Here, this work presents a plasmonic vesicle‐mediated fusogenic immunoassay (PVFIA) comprising gold nanoparticle (GNP) encapsulating fusogenic polymeric vesicles (plasmonic vesicles; PVs) for the label‐free and colorimetric detection of influenza A virus (IAV). The PVFIA combines two sequential assays: a biochip‐based immunoassay for target‐specific capture and a PV‐induced fusion assay for color change upon the IAV–PV fusion complex formation. The PVFIA demonstrates excellent specificity in capturing the target IAV, while the fusion conditions and GNP induce a significant color change, enabling visual detection. The integration of two consecutive assays results in a low detection limit (100.7919 EID50 mL−1) and good reliability (0.9901), indicating sensitivity that is 104.208 times higher than conventional immunoassay. Leveraging the PV viral membrane fusion activity renders the PVFIA promising for point‐of‐care diagnostics through colorimetric detection. The innovative approach addresses the critical need for detecting active and intact virions with infectivity, providing a valuable tool with which to combat the spread of the virus.
Funder
National Research Foundation of Korea
Ministry of Science and ICT, South Korea
Korea Environmental Industry and Technology Institute
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry
Reference46 articles.
1. Airborne transmission of respiratory viruses
2. Transmissibility and transmission of respiratory viruses
3. Culture shock: COVID‐19 and the cultural and creative sectors www.oecd.org/coronavirus/policy‐responses/culture‐shock‐covid‐19‐and‐the‐cultural‐and‐creative‐sectors‐08da9e0e/(accessed: October 2020).
4. Listings of WHO's response to COVID‐19 www.who.int/news/item/29‐06‐2020‐covidtimeline(accessed: October 2021).
5. Influenza (Seasonal) https://www.who.int/news‐room/fact‐sheets/detail/influenza‐(seasonal) (accessed: September 2018).