Affiliation:
1. Infection, Immunity, and Inflammation Research & Teaching Department UCL Great Ormond Street Institute of Child Health 30 Guilford Street London WC1N 1EH UK
2. Universal BioProducts 2151 South 30th Street Haines City FL 33844 USA
3. BioPhysics Group Institute of Biomedical Engineering Centre for Stem Cells and Regenerative Medicine and Department of Mechanical Engineering University College London Torrington London WC1E 7JE UK
Abstract
AbstractOrganotypic 3D tissue models have greatly contributed to understand a wide range of molecular and cellular characteristics within a functional or diseased tissue. Human skin reconstructs which act as models are most useful for a wide range of investigations, ranging from tissue engineering and regenerative medicine, drug development, screening, and discovery to name a few. There are many approaches for reconstructing 3D skin tissue models, however, to date there have been very few that are able to generate organotypic 3D constructs with a single technology having minimal processing steps to finally scalability. The many manifestations of 3D bioprinting have contributed to this endeavor, having said that, the technology's limitations have tempered those reconstructed models, as they are known to contain low cell numbers/concentrations to those having damaged/dead molecules/cells within the reconstructed tissue, which are not desirable, for exploring as tissues models. Contrary to 3D bioprinting approaches, bio‐electrosprays have been demonstrated to possess the ability to handle large concentrations of cells and molecules to whole fertilized embryos without damaging them from a molecular level upwards. Consequently, this article demonstrates, for the first time, bio‐electrospray's capacity to reconstruct skin‐like structures in vitro and its potential in reconstructing full‐thickness 3D organotypic human skin tissues.
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry
Cited by
4 articles.
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