Affiliation:
1. State Key Laboratory of Solidification Processing School of Materials Science and Engineering Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) Xi'an 710072 China
2. School of Engineering and Materials Science Queen Mary University of London Mile End Road London E1 4NS UK
3. Centre for Neuroscience and Brain Research Skolkovo Institute of Science and Technology Bolshoi pr.30 143025 Moscow Russia
4. School of Life Sciences Northwestern Polytechnical University Xi'an 710072 China
Abstract
Despite great advances in understanding the biological behaviors of chiral materials, the effect of chirality‐configured nanoparticles on tissue regeneration‐related biological processes remains poorly understood. Herein, the chirality of MoS2 quantum dots (QDs) is tailored by functionalization with l‐/d‐penicillamine, and the profound chiral effects of MoS2 QDs on cellular activities, angiogenesis, and tissue regeneration are thoroughly investigated. Specifically, d‐MoS2 QDs show a positive effect in promoting the growth, proliferation, and migration of human umbilical vein endothelial cells. The expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and fibroblast growth factor (FGF) in d‐MoS2 QDs group is substantially up‐regulated, resulting in enhanced tube formation activity. This distinct phenomenon is largely due to the higher internalization efficiency of d‐MoS2 QDs than l‐MoS2 QDs and chirality‐dependent nano‐bio interactions. In vivo angiogenic assay shows the expression level of angiogenic markers in newly‐formed skin tissues of d‐MoS2 QDs group is higher than that in l‐MoS2 QDs group, leading to an accelerated re‐epithelialization and improved skin regeneration. The findings of chirality‐dependent angiogenesis activity of MoS2 QDs provide new insights into the biological activity of MoS2 nanomaterials, which also opens up a new path to the rational design of chiral nanomaterials for tissue regeneration application.
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Key Research and Development Projects of Shaanxi Province
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry