Pathogen‐Activated Macrophage Membrane Encapsulated CeO2‐TCPP Nanozyme with Targeted and Photo‐Enhanced Antibacterial Therapy

Abstract

AbstractNanozymes with peroxidase‐mimic activity have recently emerged as effective strategies for eliminating infections. However, challenges in enhancing catalytic activities and the ability to target bacteria have hindered the broader application of nanozymes in bacterial infections. Herein, a novel nanozyme based on mesoporous CeO2 nanosphere and meso‐tetra(4‐carboxyphenyl)porphine (TCPP) encapsulated within pathogen‐activated macrophage membranes, demonstrates photodynamic capability coupled with photo‐enhanced chemodynamic therapy for selective and efficient antibacterial application against infected wounds. Interestingly, the expression of Toll‐like receptors accordingly upregulates when macrophages are co‐cultured with specific bacteria, thereby facilitating to recognition of the pathogen‐associated molecular patterns originating from bacteria. The CeO2 not only serve as carriers for TCPP, but also exhibit intrinsic peroxidase‐like catalytic activity. Consequently, Staphylococcus aureus (S. aureus)‐activated macrophage membrane‐coated CeO2‐TCPP (S‐MM@CeO2‐TCPP) generated singlet oxygen, and simultaneously promoted photo‐enhanced chemodynamic therapy, significantly boosting reactive oxygen species (ROS) to effectively eliminate bacteria. S‐MM@CeO2‐TCPP specifically targeted S. aureus via Toll‐like receptor, thereby directly disrupting bacterial structural integrity to eradicate S. aureus in vitro and relieve bacteria‐induced inflammation to accelerate infected wound healing in vivo. By selectively targeting specific bacteria and effectively killing pathogens, such strategy provides a more efficient and reliable alternative for precise elimination of pathogens and inflammation alleviation in microorganism‐infected wounds.