Affiliation:
1. Regional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute (CATRIN) Palacký University Olomouc Šlechtitelů 27 Olomouc 783 71 Czech Republic
2. Department of Physical Chemistry Faculty of Science Palacký University Olomouc 771 46 Czech Republic
3. Nanotechnology Centre Centre of Energy and Environmental Technologies VSB – Technical University of Ostrava 17. listopadu 2172/15 708 00 Ostrava‐Poruba Czech Republic
4. IT4Innovations VSB – Technical University of Ostrava 17. listopadu 2172/15 Ostrava‐Poruba 708 00 Czech Republic
Abstract
AbstractTackling the current problem of antimicrobial resistance (AMR) requires fast, inexpensive, and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost‐effective, disposable, point‐of‐care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon‐based materials and inks that enable effective bioconjugation under mild conditions for the development of robust, sensitive, and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chemistry. Using click chemistry, an aptamer is immobilized and used as a successful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibiotics, the storage stability of 4 weeks, and is effective in real samples. Additionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen‐printed carbon electrodes usable in point‐of‐care devices.
Funder
Grantová Agentura České Republiky
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry
Cited by
2 articles.
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