Affiliation:
1. College of Chemistry and Material Science Key Laboratory of Low‐Carbon and Green Agriculture Chemistry in Universities of Shandong Key Laboratory of Agricultural Film Application of Ministry of Agriculture and Rural Affairs Food Safety Analysis and Test Engineering Technology Research Center of Shandong Province Shandong Agricultural University Tai'an 271018 P. R. China
2. Key Laboratory of Marine Eco‐Environmental Science and Technology Marine Bioresource and Environment Research Center First Institute of Oceanography Ministry of Natural Resources Qingdao 266061 P. R. China
3. College of Information Science and Engineering Ocean University of China Qingdao 266061 P. R. China
Abstract
AbstractOxygen vacancy defects (OVs) are one of the main strategies for nanomaterials modification to improve the photoactivity, but current methods for fabricating OVs are usually complicated and harsh. It is important to develop simple, rapid, safe, and mild methods to fabricate OVs. By studying the effects of different weak reducing agents, the concentration of the reducing agent and the reaction time on fabrication of OVs, it is found that L‐ascorbic acid (AA) gently and rapidly induces the increase of OVs in Bi4O5Br2 at room temperature. The increased OVs not only improve the adsorption of visible light, but also enhance the photocurrent response. Based on this, the preparation of OVs in Bi4O5Br2 is employed to the development of a photoelectrochemical biosensor for the detection of DNA demethylase of methyl‐CpG binding domain protein 2 (MBD2). The biosensor shows a wide linear range of 0.1–400 ng mL−1 and a detection limit as low as 0.03 ng mL−1 (3σ). In addition, the effect of plasticizers on MBD2 activity is evaluated using this sensor. This work not only provides a novel method to prepare OVs in bismuth rich materials, but also explores a new novel evaluation tool for studying the ecotoxicological effects of contaminants.
Funder
National Natural Science Foundation of China
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry