Affiliation:
1. Department of Pain Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing 210008 China
2. Department of Gastroenterology Affiliated Nanjing Drum Tower Hospital Medical School of Nanjing University Nanjing 210008 China
3. State Key Laboratory of Pharmaceutical Biotechnology Medical School and School of Life Science Nanjing University Nanjing 210008 China
4. Institute of Drug R&D Medical School Nanjing University Nanjing 210008 China
Abstract
AbstractSpinal cord injury (SCI) can lead to devastating autonomic dysfunction. One of the most challenging issues for functional repair in SCI is the secondary damage caused by the increased release of glutamate and free Ca2+ from injured cells. Here, an in situ assembled trapping gel (PF‐SA‐GAD) is developed to sweep glutamate and Ca2+, promoting SCI repair. The hydrogel solution is a mixture of recombinant glutamate decarboxylase 67 (rGAD67) protein, sodium alginate (SA), and pluronic F‐127 (PF‐127). After intrathecal administration, temperature‐sensitive PF‐127 promoted in situ gelation. Glutamate (Glu) is captured and decarboxylated by rGAD67 into γ‐aminobutyric acid (GABA). SA reacted with the free Ca2+ to generate gellable calcium alginate. Thereby, this in situ trapping gel retarded secondary neuron injury caused by Glu and free Ca2+ during SCI. In rat models of SCI, PF‐SA‐GAD reduces the lesion volume and inflammatory response after SCI, restores the motor function of rats with SCI. Together, the in situ assembled trapping gel is a long‐term effective and minimally invasive sweeper for the direct elimination of glutamate and Ca2+ from injury lesions and can be a novel strategy for SCI repair by preventing secondary injury.
Funder
National Natural Science Foundation of China
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献