Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction-Reference-Cited by-同舟云学术

Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction

Published:2024-04 Issue:4 Volume:36 Page:
ISSN:0899-0042
Container-title:Chirality
language:en
Short-container-title:Chirality

Author:

Takahashi Masayuki¹^ORCID,Ito Kentaro²,Iwasaki Hiroshi¹³,Norden Bengt⁴

Affiliation:

1. School of Life Science and Technology Tokyo Institute of Technology Tokyo Japan

2. Graduate School of Medical Life Science Yokohama City University Yokohama Japan

3. Innovative Science Institute Tokyo Institute of Technology Yokohama Japan

4. Department of Chemical and Biological Engineering Chalmers University of Technology Gothenburg Sweden

Abstract

AbstractLinear dichroism spectroscopy is used to investigate the structure of RecA family recombinase filaments (RecA and Rad51 proteins) with DNA for clarifying the molecular mechanism of DNA strand exchange promoted by these proteins and its activation. The measurements show that the recombinases promote the perpendicular base orientation of single‐stranded DNA only in the presence of activators, indicating the importance of base orientation in the reaction. We summarize the results and discuss the role of DNA base orientation.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/chir.23664

Reference22 articles.

1. Linear dichroism spectroscopy of nucleic acids

2. Linear Dichroism Measurements for the Study of Protein-DNA Interactions

3. RecA: Regulation and Mechanism of a Molecular Search Engine

4. Rad51-mediated double-strand break repair and mismatch correction of divergent substrates