Transient excited states of the metamorphic protein Mad2 and their implications for function

Abstract

AbstractThe spindle checkpoint complex is a key surveillance mechanism in cell division that prevents premature separation of sister chromatids. Mad2 is an integral component of this spindle checkpoint complex that recognizes cognate substrates such as Mad1 and Cdc20 in its closed (C‐Mad2) conformation by fastening a “seatbelt” around short peptide regions that bind to the substrate recognition site. Mad2 is also a metamorphic protein that adopts not only the fold found in C‐Mad2, but also a structurally distinct open conformation (O‐Mad2) which is incapable of binding substrates. Here, we show using chemical exchange saturation transfer (CEST) and relaxation dispersion (CPMG) NMR experiments that Mad2 transiently populates three other higher free energy states with millisecond lifetimes, two in equilibrium with C‐Mad2 (E1 and E2) and one with O‐Mad2 (E3). E1 is a mimic of substrate‐bound C‐Mad2 in which the N‐terminus of one C‐Mad2 molecule inserts into the seatbelt region of a second molecule of C‐Mad2, providing a potential pathway for autoinhibition of C‐Mad2. E2 is the “unbuckled” conformation of C‐Mad2 that facilitates the triage of molecules along competing fold‐switching and substrate binding pathways. The E3 conformation that coexists with O‐Mad2 shows fluctuations at a hydrophobic lock that is required for stabilizing the O‐Mad2 fold and we hypothesize that E3 represents an early intermediate on‐pathway towards conversion to C‐Mad2. Collectively, the NMR data highlight the rugged free energy landscape of Mad2 with multiple low‐lying intermediates that interlink substrate‐binding and fold‐switching, and also emphasize the role of molecular dynamics in its function.