Simultaneous quantification of icaritin and its novel 3‐methylcarbamate prodrug in rat plasma using HPLC–MS/MS and its application to pharmacokinetic study

Abstract

AbstractA sensitive, rapid, and simple HPLC–MS/MS method was first developed and fully validated to determine the icaritin (ICT) and its novel 3‐methylcarbamate prodrug (3N) simultaneously in rat plasma. Analytes were extracted from rat plasma using a liquid–liquid extraction (LLE) method. Chromatographic separation was performed on ACE Excel 2 C18‐Amide column. Quantitation of analytes was conducted on an LCMS‐8060 triple‐quadrupole tandem mass spectrometer. The quantitation mode was the multiple reaction monitoring via positive electrospray ionization. The calibration curve was linear over the concentration range of 1 to 200 ng/ml for ICT with a correlation coefficient of r = 0.9950 and 1 to 400 ng/ml for 3N with a correlation coefficient of r = 0.9956. The intra‐precision RSDs were ≤12% for ICT and 3N. The inter‐day precision RSDs were ≤10% for ICT and 3N. The accuracy RE was between −2.6% and 7.8% for ICT and 3N. The average ICT, 3N and IS recoveries were 87.9%, 83.6%, and 84.3%. The plasma matrix of ICT and 3N complied with the guidelines. ICT and 3N were stable in rat plasma under various tested conditions. This work has been successfully applied to studying the pharmacokinetics of ICT and 3N.