Affiliation:
1. Henan Institute of Medical and Pharmaceutical Sciences Zhengzhou University Zhengzhou Henan China
2. Academy of Medical Sciences Zhengzhou University Zhengzhou Henan China
3. BGI College Zhengzhou University Zhengzhou Henan China
4. School of Basic Medical Sciences, Academy of Medical Science Zhengzhou University Zhengzhou Henan China
5. Henan Key Laboratory of Tumor Epidemiology and State Key Laboratory of Esophageal Cancer Prevention & Treatment Zhengzhou University Zhengzhou Henan Province China
Abstract
AbstractEpinodosin has shown antibacterial and antitumor biological characteristics in the documents. We found that Epinodosin has an effective inhibitory effect on esophageal squamous cell carcinoma (ESCC). However, the potential roles and mechanisms of Epinodosin in ESCC remain unclear. We performed many experiments to clarify the effect and mechanism of Epinodosin on ESCC. In this study, cell viability, invasion, migration, and apoptosis were determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,‐diphenytetrazoliumromide (MTT), Transwell, and flow cytometry. The differentially expressed miRNAs were screened through RNA transcriptome sequencing. The expression levels of miRNA‐143‐3p and some proteins were measured by real‐time polymerase chain reaction (PCR) and Western blot. The anticancer effects of Epinodosin in vivo were determined by a nude mouse model. Epinodosin suppressed cell proliferation/invasion/migration and induced ESCC cell apoptosis. Epinodosin remarkably affected the protein expression of mitogen‐activated protein kinase (MAPK) signaling pathway. The animal experiments demonstrated that Epinodosin could attenuate the growth of ESCC tumors in nude mice. The expression of p53, Bim, and Bax was upregulated, while that of Bcl‐2 was downregulated in tumor tissues. In conclusion, Epinodosin suppresses cell viability/invasion/migration, while induces ESCC cell apoptosis by mediating miRNA‐143‐3p and Bcl‐2, and can markedly attenuate the growth of ESCC tumors in nude mice.
Funder
Natural Science Foundation of Henan Province