Increased membrane Ca2+ permeability drives astrocytic Ca2+ dynamics during neuronal stimulation at excitatory synapses

Author:

Hjukse Jarand B.1,Puebla Mario F. D. L.123,Vindedal Gry Fluge1,Sprengel Rolf4,Jensen Vidar1,Nagelhus Erlend A.15ORCID,Tang Wannan123ORCID

Affiliation:

1. Department of Molecular Medicine, Institute of Basic Medical Sciences University of Oslo Oslo Norway

2. Department of Clinical and Molecular Medicine Norwegian University of Science and Technology (NTNU) Trondheim Norway

3. Department of Neurology Neuroclinic, St. Olavs Hospital Trondheim Norway

4. Department of Neurology Oslo University Hospital Oslo Norway

5. Research Group of Molecular Neurobiology Max Planck Institute for Medical Research Heidelberg Germany

Abstract

AbstractAstrocytes are intricately involved in the activity of neural circuits; however, their basic physiology of interacting with nearby neurons is not well established. Using two‐photon imaging of neurons and astrocytes during higher frequency stimulation of hippocampal CA3‐CA1 Schaffer collateral (Scc) excitatory synapses, we could show that increasing levels of released glutamate accelerated local astrocytic Ca2+ elevation. However, blockage of glutamate transporters did not abolish this astrocytic Ca2+ response, suggesting that astrocytic Ca2+ elevation is indirectly associated with an uptake of extracellular glutamate. However, during the astrocytic glutamate uptake, the Na+/Ca2+ exchanger (NCX) reverse mode was activated, and mediated extracellular Ca2+ entry, thereby triggering the internal release of Ca2+. In addition, extracellular Ca2+ entry via membrane P2X receptors further facilitated astrocytic Ca2+ elevation via ATP binding. These findings suggest a novel mechanism of activity induced Ca2+ permeability increases of astrocytic membranes, which drives astrocytic responses during neuronal stimulation of CA3‐CA1 Scc excitatory synapses.

Funder

Letten Foundation

Publisher

Wiley

Subject

Cellular and Molecular Neuroscience,Neurology

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