Schisandra chinensis (Turcz.) Baill. polysaccharide inhibits influenza A virus in vitro and in vivo

Author:

Qi Jing12,Lv Chaoxiang23,Guo Jin24,Li Yuanguo2,Sima Mingwei25,Luo Rongbo2,Xiang Haiyang2,Xia Xianzhu2,Zhou Yifa1,Wang Tiecheng2ORCID

Affiliation:

1. College of Life Sciences Northeast Normal University Changchun China

2. Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences China

3. The Research Center for Preclinical Medicine Southwest Medical University Luzhou China

4. College of Life Sciences Shandong Normal University Jinan China

5. College of Basic Medicine Changchun University of Chinese Medicine China

Abstract

Influenza virus is prone to seasonal spread and widespread outbreaks, which pose important challenges to public health security. Therefore, it is important to effectively prevent and treat influenza virus infection. Schisandra polysaccharide (SPJ) is a polysaccharide derived from the fruit of Schisandra chinensis (Turcz.) Baill. In this study, we evaluated the antiviral activity of SPJ in vitro and in vivo, especially against influenza A virus (IAV) infection. By analyzing SPJ structure and monosaccharide composition, the molecular weight of SPJ was determined to be 115.5 KD, and it is composed of galacturonic acid (89.4%), rhamnose (0.8%), galactose (4.4%), arabinose (3.8%), and glucose (1.7%). Immunofluorescence analysis showed that SPJ treatment reduced the positive rate of viral nucleoproteins in cells, indicating that the compound had an inhibitory effect on influenza virus replication. Furthermore, SPJ therapy improved the survival of infected mice. Lung virus titer assays indicated that SPJ treatment significantly reduced viral loading in the lung tissue of infected mice and alleviated the pathological damage caused by influenza virus infection. Moreover, SPJ reduced cytokine expression during influenza virus challenge. In conclusion, SPJ has anti‐influenza virus effects and may have potential as an anti‐influenza drug candidate in further clinical studies.

Publisher

Wiley

Subject

General Biochemistry, Genetics and Molecular Biology

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