Mitochondrial ATP concentration decreases immediately after glucose administration to glucose‐deprived hepatocytes

Author:

Tsuno Saki12ORCID,Harada Kazuki1ORCID,Horikoshi Mina3,Mita Marie1ORCID,Kitaguchi Tetsuya4ORCID,Hirai Masami Yokota5ORCID,Matsumoto Mitsuharu2ORCID,Tsuboi Takashi13ORCID

Affiliation:

1. Department of Life Sciences, Graduate School of Arts and Sciences The University of Tokyo Tokyo Japan

2. Dairy Science and Technology Institute Kyodo Milk Industry Co., Ltd. Tokyo Japan

3. Department of Biological Sciences, Graduate School of Science The University of Tokyo Tokyo Japan

4. Laboratory for Chemistry and Life Science, Institute of Innovative Research Tokyo Institute of Technology Yokohama Japan

5. RIKEN Center for Sustainable Resource Science Yokohama Japan

Abstract

Hepatocytes can switch their metabolic processes in response to nutrient availability. However, the dynamics of metabolites (such as lactate, pyruvate, and ATP) in hepatocytes during the metabolic switch remain unknown. In this study, we visualized metabolite dynamics in primary cultured hepatocytes during recovery from glucose‐deprivation. We observed a decrease in the mitochondrial ATP concentration when glucose was administered to hepatocytes under glucose‐deprivation conditions. In contrast, there was slight change in the cytoplasmic ATP concentration. A decrease in mitochondrial ATP concentration was associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species. These results suggest that mitochondrial ATP is important in switching metabolic processes in the hepatocytes.

Funder

Japan Society for the Promotion of Science

Publisher

Wiley

Subject

General Biochemistry, Genetics and Molecular Biology

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