Affiliation:
1. School of Integrative Medicine Tianjin University of Traditional Chinese Medicine Tianjin People's Republic of China
2. Oncology Department Ganzhou People's Hospital Ganzhou People's Republic of China
Abstract
AbstractEndothelial pyroptosis promotes cerebral ischemia/reperfusion injury (CIRI). Sodium Danshensu (SDSS) has been shown to attenuate CIRI and have anti‐inflammatory properties in endothelial cells. However, the mechanism and effect of SDSS on alleviating endothelial pyroptosis after CIRI remains poorly understood. Thus, we aimed to investigate the efficacy and mechanism of SDSS in reducing endothelial pyroptosis. It has been shown that SDSS administration inhibited NLRP3 inflammasome‐mediated pyroptosis. As demonstrated by protein microarrays, molecular docking, CETSA and ITDRFCETSA, SDSS bound strongly to CLIC4. Furthermore, SDSS can decrease its expression and inhibit its translocation. Its effectiveness was lowered by CLIC4 overexpression but not by knockdown. Overall The beneficial effect of SDSS against CIRI in this study can be ascribed to blocking endothelial pyroptosis by binding to CLIC4 and then inhibiting chloride efflux‐dependent NLRP3 inflammasome activation.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Tianjin City
Subject
Clinical Biochemistry,Molecular Medicine,General Medicine,Biochemistry