Affiliation:
1. Department of Nephrology, Nanjing Drum Tower Hospital Medical School of Nanjing University Nanjing Jiangsu China
Abstract
AbstractBackgroundPeritoneal fibrosis is a common complication of peritoneal dialysis, which may lead to ultrafiltration failure and ultimately treatment discontinuation. LncRNAs participate in many biological processes during tumorigenesis. We investigated the role of AK142426 in peritoneal fibrosis.MethodsThe AK142426 level in peritoneal dialysis (PD) fluid was detected by quantitative real‐time‐PCR assay. The M2 macrophage distribution was determined by flow cytometry. The inflammatory cytokines of TNF‐α and TGF‐β1 were measured by ELISA assay. The direct interaction between AK142426 and c‐Jun was evaluated by RNA pull‐down assay. In addition, the c‐Jun and fibrosis related proteins were assessed by western blot analysis.ResultsThe PD‐induced peritoneal fibrosis mouse model was successfully established. More importantly, PD treatment induced M2 macrophage polarization and the inflammation in PD fluid, which might be associated with exosome transmission. Fortunately, AK142426 was observed to be upregulated in PD fluid. Mechanically, knockdown of AK142426 suppressed M2 macrophage polarization and inflammation. Furthermore, AK142426 could upregulate c‐Jun through binding c‐Jun protein. In rescue experiments, overexpression of c‐Jun could partially abolish the inhibitory effect of sh‐AK142426 on the activation of M2 macrophages and inflammation. Consistently, knockdown of AK142426 alleviated peritoneal fibrosis in vivo.ConclusionsThis study demonstrated that knockdown of AK142426 suppressed M2 macrophage polarization and inflammation in peritoneal fibrosis via binding to c‐Jun, suggesting that AK142426 might be a promising therapeutic target for patients of peritoneal fibrosis.
Funder
National Natural Science Foundation of China
Subject
Genetics (clinical),Drug Discovery,Genetics,Molecular Biology,Molecular Medicine
Cited by
2 articles.
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