MYC‐activated LINC00607 promotes hepatocellular carcinoma progression by regulating the miR‐584‐3p/ROCK1 axis

Author:

Dong Shuilin12,Wang Wei12,Liao Zhibin12,Fan Yawei12,Wang Qi12,Zhang Lei123

Affiliation:

1. Hepatic Surgery Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei China

2. Hubei Key Laboratory of Hepato‐Pancreato‐Biliary Diseases Wuhan Hubei China

3. Department of Hepatobiliary Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Shanxi Medical University; Shanxi Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology Taiyuan China

Abstract

AbstractBackgroundThere have been many reports of long non‐coding RNAs (lncRNAs) in tumors, and abnormally expressed lncRNA is closely related to hepatocellular carcinoma (HCC). The mechanism of LINC00607 in HCC has not been reported.MethodsWe utilized qPCR to evaluate the RNA expression level. The mechanism of MYC binding to the LINC00607 promoter was revealed through chromatin immunoprecipitation assay and dual luciferase reporter assay. The proliferation and invasive ability were evaluated by CCK‐8 and transwell assays. The relation between LINC00607 and miR‐584‐3p was assessed by RNA immunoprecipitation assay and dual luciferase reporter assay. The level of ROCK1 was evaluated by qPCR and western blot.ResultsIn this research, we found that the expression of LINC00607 was higher in HCC tissues when compared with that in the adjacent non‐tumor tissues. Meanwhile, MYC was observed to interact with the LINC00607 promoter, leading to the upregulation of LINC00607 in HCC. We further revealed that LINC00607 functioned as a sponge for miR‐584‐3p. Cell proliferation and migration assays showed that miR‐584‐3p may inhibit the HCC progression. Moreover, we found that the miR‐584‐3p inhibitor could reverse the effects of LINC00607 downregulation in HCC through rescue experiments. Through verification, miR‐584‐3p bound to the 3′ UTR of ROCK1 to downregulate its expression.ConclusionLINC00607 regulated by MYC can promote the proliferation, migration and invasion of HCC cells through the miR‐584‐3p/ROCK1 axis.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Genetics (clinical),Drug Discovery,Genetics,Molecular Biology,Molecular Medicine

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