Hydrophilic cryogels as potential 3D cell culture materials: Synthesis and characterization of 2‐(methacrylamido) glucopyranose‐based polymer scaffolds

Author:

Behrendt Florian12,Pretzel David123,Cseresnyés Zoltán4,Kleinsteuber Maximilian12,Wloka Thomas12,Radosa Lukáš5,Figge Marc Thilo46,Gottschaldt Michael12,Brakhage Axel56,Schubert Ulrich S.123ORCID

Affiliation:

1. Laboratory of Organic and Macromolecular Chemistry (IOMC) Friedrich Schiller University Jena Jena Germany

2. Jena Center for Soft Matter (JCSM) Friedrich Schiller University Jena Jena Germany

3. Abbe Center of Photonics (ACP) Friedrich Schiller University Jena Jena Germany

4. Applied Systems Biology Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI) Jena Germany

5. Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI) Jena Germany

6. Institute of Microbiology Faculty of Biological Sciences, Friedrich Schiller University Jena Jena Germany

Abstract

AbstractCell cultures are important techniques to investigate cellular processes in a simplified manner. The most commonly applied 2D models do not simulate most of the in vivo conditions whereas cryogels as promising 3D cell culture materials are able to provide both a structured microenvironment and tailored surface properties, similar to the natural extracellular matrix (ECM). Herein, we present the synthesis and characterization of hydrophilic cryogels with different 2‐(methacrylamido) glucopyranose amounts followed by 3D cell culture experiments with L929 cells. Selected samples are additionally functionalized with fibronectin‐FITC for improved cellular adhesion. The incorporation of different 2‐(methacrylamido) glucopyranose amounts into the cryogels is demonstrated by Fourier‐transform IR (FTIR) and solid‐state NMR (ssNMR). The gels are able to withstand harsh autoclaving conditions as shown by thermogravimetric analysis (TGA) and ssNMR measurements. Pore size measurements by automated scanning electron microscopy (SEM) image analysis reveal similar pore sizes among the entire cryogel series. Confocal laser scanning microscopy (CLSM) images suggest the presence of healthy cells due to the absence of morphological abnormalities among the entire cryogel series. L929 cells settled deeper into the cryogels with increasing MAG content. With additional fibronectin functionalization, the cells are retained much stronger and stay in the upper gel layers.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Materials Chemistry,Polymers and Plastics,Physical and Theoretical Chemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3