Affiliation:
1. Department of General Surgery The First Affiliated Hospital of Anhui Medical University Hefei Anhui China
2. Department of Pharmacy The First Affiliated Hospital of Anhui Medical University Hefei Anhui China
3. Department of Clinical Medicine, The First Clinical College Anhui Medical University Hefei Anhui China
4. Department of Geriatric Surgery, The First Hospital China Medical University Shenyang China
Abstract
AbstractThe poor prognosis of pancreatic ductal adenocarcinoma (PDAC) is attribute to the aggressive local invasion, distant metastasis and drug resistance of PDAC patients, which was strongly accelerated by epithelial–mesenchymal transition (EMT). In current study, we systematically investigate the role of ZNF263/RNF126 axis in the initiation of EMT in PDAC in vitro and vivo. ZNF263 is firstly identified as a novel transactivation factor of RNF126. Both ZNF263 and RNF126 were overexpressed in PDAC tissues, which were associated with multiple advanced clinical stages and poor prognosis of PDAC patients. ZNF263 overexpression promoted cell proliferation, drug resistance and EMT in vitro via activating RNF126 following by the upregulation of Cyclin D1, N‐cad, and MMP9, and the downregulation of E‐cad, p21, and p27. ZNF263 silencing contributed to the opposite phenotype. Mechanistically, ZNF263 transactivated RNF126 via binding to its promoter. Further investigations revealed that ZNF263 interacted with ZNF31 to coregulate the transcription of RNF126, which in turn promoted ubiquitination‐mediated degradation of PTEN. The downregulation of PTEN activated AKT/Cyclin D1 and AKT/GSK‐3β/β‐catenin signaling, thereby promoting the malignant phenotype of PDAC. Finally, the coordination of ZNF263 and RNF126 promotes subcutaneous tumor size and distant liver metastasis in vivo. ZNF263, as an oncogene, promotes proliferation, drug resistance and EMT of PDAC through transactivating RNF126.