Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author:

Cossarizza Andrea1,Chang Hyun‐Dong2,Radbruch Andreas2,Acs Andreas3,Adam Dieter4,Adam‐Klages Sabine5,Agace William W.67,Aghaeepour Nima8,Akdis Mübeccel9,Allez Matthieu10,Almeida Larissa Nogueira11,Alvisi Giorgia12,Anderson Graham13,Andrä Immanuel14,Annunziato Francesco15,Anselmo Achille16,Bacher Petra417,Baldari Cosima T.18,Bari Sudipto1920,Barnaba Vincenzo212223,Barros‐Martins Joana24,Battistini Luca25,Bauer Wolfgang26,Baumgart Sabine2,Baumgarth Nicole27,Baumjohann Dirk28,Baying Bianka29,Bebawy Mary30,Becher Burkhard3132,Beisker Wolfgang33,Benes Vladimir29,Beyaert Rudi34,Blanco Alfonso35,Boardman Dominic A.3637,Bogdan Christian3839,Borger Jessica G.40,Borsellino Giovanna41,Boulais Philip E.4243,Bradford Jolene A.44,Brenner Dirk454647,Brinkman Ryan R.4849,Brooks Anna E. S.50,Busch Dirk H.145152,Büscher Martin53,Bushnell Timothy P.54,Calzetti Federica55,Cameron Garth56,Cammarata Ilenia21,Cao Xuetao57,Cardell Susanna L.58,Casola Stefano59,Cassatella Marco A.55,Cavani Andrea60,Celada Antonio61,Chatenoud Lucienne62,Chattopadhyay Pratip K.63,Chow Sue64,Christakou Eleni6566,Čičin‐Šain Luka67,Clerici Mario686970,Colombo Federico S.16,Cook Laura3771,Cooke Anne72,Cooper Andrea M.73,Corbett Alexandra J.56,Cosma Antonio74,Cosmi Lorenzo15,Coulie Pierre G.75,Cumano Ana76,Cvetkovic Ljiljana77,Dang Van Duc2,Dang‐Heine Chantip78,Davey Martin S.7980,Davies Derek81,De Biasi Sara82,Del Zotto Genny83,Dela Cruz Gelo Victoriano84,Delacher Michael8586,Della Bella Silvia87,Dellabona Paolo88,Deniz Günnur89,Dessing Mark90,Di Santo James P.9192,Diefenbach Andreas29394,Dieli Francesco95,Dolf Andreas96,Dörner Thomas297,Dress Regine J.98,Dudziak Diana99,Dustin Michael100,Dutertre Charles‐Antoine10198,Ebner Friederike102,Eckle Sidonia B. G.56,Edinger Matthias85103,Eede Pascale104,Ehrhardt Götz R.A.105,Eich Marcus106,Engel Pablo107,Engelhardt Britta108,Erdei Anna109,Esser Charlotte110,Everts Bart111,Evrard Maximilien98,Falk Christine S.112,Fehniger Todd A.113,Felipo‐Benavent Mar114,Ferry Helen115,Feuerer Markus8586,Filby Andrew116,Filkor Kata117,Fillatreau Simon118,Follo Marie119120,Förster Irmgard121,Foster John122,Foulds Gemma A.123,Frehse Britta11,Frenette Paul S.4243124,Frischbutter Stefan2125,Fritzsche Wolfgang126,Galbraith David W.127128,Gangaev Anastasia129,Garbi Natalio130,Gaudilliere Brice131,Gazzinelli Ricardo T.132133,Geginat Jens134,Gerner Wilhelm135136,Gherardin Nicholas A.56,Ghoreschi Kamran137,Gibellini Lara82,Ginhoux Florent98138139,Goda Keisuke140141142,Godfrey Dale I.56,Goettlinger Christoph143,González‐Navajas Jose M.144145,Goodyear Carl S.146,Gori Andrea147,Grogan Jane L.148,Grummitt Daryl122,Grützkau Andreas2,Haftmann Claudia31,Hahn Jonas149,Hammad Hamida150,Hämmerling Günter151,Hansmann Leo94152153,Hansson Goran154,Harpur Christopher M.155,Hartmann Susanne102,Hauser Andrea103,Hauser Anja E.2156157,Haviland David L.158,Hedley David64,Hernández Daniela C.2159,Herrera Guadalupe160,Herrmann Martin149,Hess Christoph161162,Höfer Thomas163,Hoffmann Petra85103,Hogquist Kristin164,Holland Tristan130,Höllt Thomas165166,Holmdahl Rikard167,Hombrink Pleun168169,Houston Jessica P.170,Hoyer Bimba F.171,Huang Bo172,Huang Fang‐Ping173,Huber Johanna E.28,Huehn Jochen174,Hundemer Michael175,Hunter Christopher A.176,Hwang William Y. K.17720178,Iannone Anna179,Ingelfinger Florian31,Ivison Sabine M3637,Jäck Hans‐Martin77,Jani Peter K.2180,Jávega Beatriz181,Jonjic Stipan182,Kaiser Toralf2,Kalina Tomas183,Kamradt Thomas184,Kaufmann Stefan H. E.180,Keller Baerbel185186,Ketelaars Steven L. C.129,Khalilnezhad Ahad98187,Khan Srijit105,Kisielow Jan188,Klenerman Paul115,Knopf Jasmin149,Koay Hui‐Fern56,Kobow Katja189,Kolls Jay K.190,Kong Wan Ting98,Kopf Manfred188,Korn Thomas191,Kriegsmann Katharina175,Kristyanto Hendy192,Kroneis Thomas193,Krueger Andreas194,Kühne Jenny112,Kukat Christian195,Kunkel Désirée196197,Kunze‐Schumacher Heike194,Kurosaki Tomohiro198,Kurts Christian130,Kvistborg Pia129,Kwok Immanuel98199,Landry Jonathan29,Lantz Olivier200,Lanuti Paola201,LaRosa Francesca6870,Lehuen Agnès202,LeibundGut‐Landmann Salomé203,Leipold Michael D.204,Leung Leslie Y.T.105,Levings Megan K.3637205,Lino Andreia C.297,Liotta Francesco15,Litwin Virginia206,Liu Yanling105,Ljunggren Hans‐Gustaf207,Lohoff Michael208,Lombardi Giovanna209,Lopez Lilly210,López‐Botet Miguel211,Lovett‐Racke Amy E.212,Lubberts Erik213,Luche Herve214,Ludewig Burkhard215,Lugli Enrico1216,Lunemann Sebastian216,Maecker Holden T.217,Maggi Laura15,Maguire Orla218,Mair Florian219,Mair Kerstin H.135136,Mantovani Alberto220221,Manz Rudolf A.11,Marshall Aaron J.222,Martínez‐Romero Alicia223,Martrus Glòria216,Marventano Ivana6870,Maslinski Wlodzimierz224,Matarese Giuseppe225,Mattioli Anna Vittoria82226,Maueröder Christian227228,Mazzoni Alessio15,McCluskey James56,McGrath Mairi2,McGuire Helen M.229,McInnes Iain B.146,Mei Henrik E.2,Melchers Fritz2180,Melzer Susanne230,Mielenz Dirk77,Miller Stephen D.231,Mills Kingston H.G.232,Minderman Hans218,Mjösberg Jenny207233,Moore Jonni234,Moran Barry232,Moretta Lorenzo235,Mosmann Tim R.236,Müller Susann237,Multhoff Gabriele238239,Muñoz Luis Enrique149,Münz Christian3132,Nakayama Toshinori240,Nasi Milena82,Neumann Katrin241,Ng Lai Guan98187199242243,Niedobitek Antonia2,Nourshargh Sussan244,Núñez Gabriel245,O'Connor José‐Enrique181,Ochel Aaron241,Oja Anna169,Ordonez Diana246,Orfao Alberto247,Orlowski‐Oliver Eva248,Ouyang Wenjun249,Oxenius Annette250,Palankar Raghavendra251,Panse Isabel2,Pattanapanyasat Kovit252,Paulsen Malte246,Pavlinic Dinko29,Penter Livius153,Peterson Pärt253,Peth Christian53,Petriz Jordi254,Piancone Federica6870,Pickl Winfried F.255,Piconese Silvia2123,Pinti Marcello256,Pockley A. Graham123257,Podolska Malgorzata Justyna149258,Poon Zhiyong177,Pracht Katharina77,Prinz Immo24,Pucillo Carlo E. M.259,Quataert Sally A.236,Quatrini Linda235,Quinn Kylie M.260261,Radbruch Helena104,Radstake Tim R. D. J.262,Rahmig Susann263,Rahn Hans‐Peter264,Rajwa Bartek265,Ravichandran Gevitha241,Raz Yotam266,Rebhahn Jonathan A.236,Recktenwald Diether267,Reimer Dorothea77,Reis e Sousa Caetano268,Remmerswaal Ester B.M.168269,Richter Lisa270,Rico Laura G.254,Riddell Andy81,Rieger Aja M.271,Robinson J. Paul272,Romagnani Chiara2159,Rubartelli Anna273,Ruland Jürgen274,Saalmüller Armin135,Saeys Yvan275276,Saito Takashi277,Sakaguchi Shimon198,Sala‐de‐Oyanguren Francisco278,Samstag Yvonne279,Sanderson Sharon280,Sandrock Inga24,Santoni Angela281,Sanz Ramon Bellmàs112,Saresella Marina6870,Sautes‐Fridman Catherine282,Sawitzki Birgit283,Schadt Linda3132,Scheffold Alexander4,Scherer Hans U.192,Schiemann Matthias14,Schildberg Frank A.284,Schimisky Esther285,Schlitzer Andreas286,Schlosser Josephine102,Schmid Stephan287,Schmitt Steffen288,Schober Kilian14,Schraivogel Daniel289,Schuh Wolfgang77,Schüler Thomas290,Schulte Reiner291,Schulz Axel Ronald2,Schulz Sebastian R.77,Scottá Cristiano209,Scott‐Algara Daniel292,Sester David P.293,Shankey T. Vincent294,Silva‐Santos Bruno295,Simon Anna Katharina100,Sitnik Katarzyna M.67,Sozzani Silvano296,Speiser Daniel E.297,Spidlen Josef298,Stahlberg Anders299,Stall Alan M.300,Stanley Natalie8,Stark Regina168169,Stehle Christina2159,Steinmetz Tobit77,Stockinger Hannes301,Takahama Yousuke302,Takeda Kiyoshi198,Tan Leonard98187,Tárnok Attila303304305,Tiegs Gisa241,Toldi Gergely117,Tornack Julia2306,Traggiai Elisabetta307,Trebak Mohamed308,Tree Timothy I.M.6566,Trotter Joe300,Trowsdale John72,Tsoumakidou Maria309,Ulrich Henning310,Urbanczyk Sophia77,Veen Willem9311,Broek Maries3132,Pol Edwin312,Van Gassen Sofie275276,Van Isterdael Gert313,Lier René A.W.169,Veldhoen Marc295,Vento‐Asturias Salvador130,Vieira Paulo76,Voehringer David314,Volk Hans‐Dieter315,Borstel Anouk7980,Volkmann Konrad316,Waisman Ari317,Walker Rachael V.318,Wallace Paul K.319,Wang Sa A.320,Wang Xin M.321,Ward Michael D.44,Ward‐Hartstonge Kirsten A36,Warnatz Klaus185186,Warnes Gary322,Warth Sarah197,Waskow Claudia263323,Watson James V.324,Watzl Carsten325,Wegener Leonie53,Weisenburger Thomas3,Wiedemann Annika297,Wienands Jürgen326,Wilharm Anneke24,Wilkinson Robert John327328329,Willimsky Gerald330,Wing James B.198,Winkelmann Rieke4,Winkler Thomas H.3,Wirz Oliver F.9,Wong Alicia98,Wurst Peter331,Yang Jennie H. M.6566,Yang Juhao174,Yazdanbakhsh Maria111,Yu Liping332,Yue Alice333,Zhang Hanlin100,Zhao Yi334,Ziegler Susanne Maria216,Zielinski Christina51335336,Zimmermann Jakob337,Zychlinsky Arturo180

Affiliation:

1. Department of Medical and Surgical Sciences for Children and AdultsUniv. of Modena and Reggio Emilia School of Medicine Modena Italy

2. Deutsches Rheuma‐Forschungszentrum (DRFZ)an Institute of the Leibniz Association Berlin Germany

3. Department of BiologyNikolaus‐Fiebiger‐Center for Molecular MedicineFriedrich‐Alexander‐University Erlangen‐Nuremberg Erlangen Germany

4. Institut für ImmunologieChristian‐Albrechts‐Universität zu Kiel Kiel Germany

5. Institut für TransfusionsmedizinUniversitätsklinik Schleswig‐Holstein Kiel Germany

6. Mucosal Immunology groupDepartment of Health TechnologyTechnical University of Denmark Kgs. Lyngby Denmark

7. Immunology SectionLund University Lund Sweden

8. Departments of Anesthesiology, Pain and Perioperative MedicineBiomedical Data Sciences; and PediatricsStanford University Stanford CA USA

9. Swiss Institute of Allergy and Asthma Research (SIAF)University of Zurich Davos Switzerland

10. Université de Paris, Institut de Recherche Saint‐Louis, INSERM U1160, and Gastroenterology DepartmentHôpital Saint‐Louis – APHP Paris France

11. Institute for Systemic Inflammation ResearchUniversity of Luebeck Luebeck Germany

12. Laboratory of Translational ImmunologyHumanitas Clinical and Research Center Rozzano Italy

13. University of Birmingham Birmingham UK

14. Institut für Medizinische MikrobiologieImmunologie und HygieneTechnische Universität München Munich Germany

15. Department of Experimental and Clinical MedicineUniversity of Florence Florence Italy

16. Flow Cytometry CoreHumanitas Clinical and Research Center Milan Italy

17. Institut für Klinische MolekularbiologieChristian‐Albrechts Universität zu Kiel Germany

18. Department of Life SciencesUniversity of Siena Siena Italy

19. Division of Medical SciencesNational Cancer Centre Singapore Singapore

20. Cancer & Stem Cell BiologyDuke-NUS Medical School Singapore

21. Dipartimento di Medicina Interna e Specialità MedicheSapienza Università di Roma Rome Italy

22. Center for Life Nano Science@SapienzaIstituto Italiano di Tecnologia Rome Italy

23. Istituto Pasteur ‐ Fondazione Cenci Bolognetti Rome Italy

24. Institute of ImmunologyHannover Medical School Hannover Germany

25. IRCCS Fondazione Santa Lucia Rome Italy

26. Division of ImmunologyAllergy and Infectious DiseasesDepartment of DermatologyMedical University of Vienna Vienna Austria

27. Center for Comparative Medicine & Dept. PathologyMicrobiology & ImmunologyUniversity of California Davis CA USA

28. Institute for ImmunologyFaculty of MedicineBiomedical Center, LMU Munich, Planegg‐Martinsried Germany

29. Genomics Core FacilityEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany

30. Discipline of PharmacyGraduate School of HealthThe University of Technology Sydney Sydney NSW Australia

31. Institute of Experimental ImmunologyUniversity of Zurich Zurich Switzerland

32. Comprehensive Cancer Center Zurich Switzerland

33. Flow Cytometry Laboratory, Institute of Molecular Toxicology and PharmacologyHelmholtz Zentrum München, German Research Center for Environmental Health München Germany

34. Department of Biomedical Molecular BiologyCenter for Inflammation ResearchGhent University ‐ VIB Ghent Belgium

35. Flow Cytometry Core TechnologiesUCD Conway InstituteUniversity College Dublin Dublin Ireland

36. Department of SurgeryThe University of British Columbia Vancouver Canada

37. BC Children's Hospital Research Institute Vancouver Canada

38. Mikrobiologisches Institut ‐ Klinische MikrobiologieImmunologie und Hygiene, Universitätsklinikum Erlangen Erlangen Germany

39. Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg and Medical Immunology Campus Erlangen Erlangen Germany

40. Department of Immunology and PathologyMonash University Melbourne Victoria Australia

41. Neuroimmunology and Flow Cytometry UnitsFondazione Santa Lucia IRCCS Rome Italy

42. Department of Cell BiologyAlbert Einstein College of Medicine Bronx NY USA

43. The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research Bronx New York USA

44. Thermo Fisher Scientific Eugene Oregon USA

45. Luxembourg Institute of HealthDepartment of Infection and ImmunityExperimental and Molecular Immunology Esch‐sur‐Alzette Luxembourg

46. Odense University Hospital, Odense Research Center for Anaphylaxis, University of Southern Denmark, Department of Dermatology and Allergy Center Odense Denmark

47. Luxembourg Centre for Systems Biomedicine (LCSB)University of Luxembourg Belvaux Luxembourg

48. Department of Medical GeneticsUniversity of British Columbia Vancouver BC Canada

49. Terry Fox LaboratoryBC Cancer Vancouver BC Canada

50. University of AucklandSchool of Biological Sciences, Maurice Wilkins Center Auckland New Zealand

51. German Center for Infection Research (DZIF) Munich Germany

52. Focus Group ‘‘Clinical Cell Processing and Purification”Institute for Advanced StudyTechnische Universität München Munich Germany

53. BiophysicsR&D EngineeringMiltenyi Biotec GmbH Bergisch Gladbach Germany

54. Department of Pediatrics and Shared Resource LaboratoriesUniversity of Rochester Medical Center Rochester NY USA

55. University of VeronaDepartment of MedicineSection of General Pathology Verona Italy

56. Department of Microbiology and ImmunologyPeter Doherty Institute for Infection and ImmunityUniversity of Melbourne Parkville Victoria Australia

57. National Key Laboratory of Medical ImmunologyNankai University Tianjin China

58. Department of Microbiology and ImmunologyUniversity of Gothenburg Gothenburg Sweden

59. The FIRC Institute of Molecular Oncology (FOM) Milan Italy

60. National Institute for HealthMigration and Poverty (INMP) Rome Italy

61. Macrophage Biology GroupSchool of BiologyUniversity of Barcelona Barcelona Spain

62. Université Paris DescartesInstitut National de la Santé et de la Recherche Médicale Paris France

63. Isaac and Laura Perlmutter Cancer CenterNYU‐Langone Medical Center New York USA

64. Divsion of Medical Oncology and HematologyPrincess Margaret Hospital Toronto Ontario Canada

65. Department of ImmunobiologySchool of Immunology and Microbial SciencesKing's College London UK

66. National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health ServiceFoundation Trust and King's College London UK

67. Department of Vaccinology and Applied MicrobiologyHelmholtz Centre for Infection Research Braunschweig Germany

68. IRCCS Fondazione Don Carlo Gnocchi Milan Italy

69. Department of Physiopathology and TransplantsUniversity of Milan Milan Italy

70. Milan Center for NeuroscienceUniversity of Milano‐Bicocca Milan Italy

71. Department of MedicineThe University of British Columbia Vancouver Canada

72. Department of PathologyUniversity of Cambridge Cambridge UK

73. Department of Respiratory SciencesUniversity of Leicester Leicester UK

74. National Cytometry PlatformLuxembourg Institute of HealthDepartment of Infection and ImmunityEsch‐sur‐Alzette Luxembourg

75. de Duve InstituteUniversité catholique de Louvain Brussels Belgium

76. Unit LymphopoiesisDepartment of ImmunologyInstitut Pasteur Paris France

77. Division of Molecular ImmunologyNikolaus‐Fiebiger‐CenterDept. of Internal Medicine IIIUniversity of Erlangen‐Nuremberg Erlangen Germany

78. Clinical Research UnitBerlin Institute of Health (BIH)Charite Universitätsmedizin Berlin Berlin Germany

79. Infection and Immunity Program and Department of Biochemistry and Molecular BiologyBiomedicine Discovery InstituteMonash University Clayton Victoria Australia

80. Australian Research Council Centre of Excellence in Advanced Molecular ImagingMonash University Clayton Victoria Australia

81. Flow Cytometry Scientific Technology PlatformThe Francis Crick Institute London UK

82. Department of SurgeryMedicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia Modena Italy

83. IRCCS Istituto Giannina Gaslini Genova Italy

84. Novo Nordisk Foundation Center for Stem Cell Biology – DanStemUniversity of Copenhagen Copenhagen Denmark

85. Regensburg Center for Interventional Immunology (RCI) Regensburg Germany

86. Chair for ImmunologyUniversity Regensburg Germany

87. Department of Medical Biotechnologies and Translational MedicineUniversity of Milan Milan Italy

88. Division of ImmunologyTransplantation and Infectious DiseasesSan Raffaele Scientific Institute Milan Italy

89. Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology Istanbul Turkey

90. Sony Europe Ltd Weybridge UK

91. Innate Immunty UnitDepartment of ImmunologyInstitut Pasteur Paris France

92. Institut Pasteur Inserm U1223 Paris France

93. Charité ‐ Universitätsmedizin BerlinLaboratory of Innate ImmunityDepartment of MicrobiologyInfectious Diseases and Immunology Berlin Germany

94. Berlin Institute of Health (BIH) Berlin Germany

95. University of PalermoCentral Laboratory of Advanced Diagnosis and Biomedical ResearchDepartment of BiomedicineNeurosciences and Advanced Diagnostics Palermo Italy

96. Flow Cytometry Core FacilityInstitute of Experimental ImmunologyUniversity of Bonn Bonn Germany

97. Dept. Medicine/Rheumatology and Clinical ImmunologyCharité Universitätsmedizin Berlin Germany

98. Singapore Immunology Network (SIgN)A*STAR (Agency for Science, Technology and Research) Biopolis Singapore

99. Department of DermatologyLaboratory of Dendritic Cell BiologyFriedrich‐Alexander Universität Erlangen‐Nürnberg (FAU)University Hospital Erlangen Erlangen Germany

100. Kennedy Institute of RheumatologyUniversity of Oxford Oxford UK

101. Program in Emerging Infectious DiseaseDuke‐NUS Medical School Singapore

102. Institute of ImmunologyCentre for Infection MedicineDepartment of Veterinary MedicineFreie Universität Berlin Germany

103. Department of Internal Medicine IIIUniversity Hospital Regensburg Germany

104. Charité – Universitätsmedizin Berlincorporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology Germany

105. Department of ImmunologyUniversity of Toronto Toronto ON Canada

106. Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI‐STEM gGmbH) Heidelberg Germany

107. University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences Barcelona Spain

108. Theodor Kocher InstituteUniversity of Bern Bern Switzerland

109. Department of ImmunologyUniversity L. Eotvos Budapest Hungary

110. Leibniz Research Institute for Environmental Medicine Düsseldorf Germany

111. Department of ParasitologyLeiden University Medical Center Leiden The Netherlands

112. Institute of Transplant ImmunologyHannover Medical SchoolMHH Hannover Germany

113. Division of OncologyWashington University School of Medicine St. Louis MO USA

114. Laboratory of CytomicsJoint Research Unit CIPF‐UVEGPrincipe Felipe Research Center Valencia Spain

115. Experimental Medicine DivisionNuffield Department of MedicineUniversity of Oxford Oxford UK

116. The Flow Cytometry Core FacilityFaculty of Medical SciencesNewcastle University Newcastle upon Tyne UK

117. MDQuest Ltd. Szeged Hungary

118. Institut Necker‐Enfants MaladesUniversité Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP‐HP, Hôpital Necker Enfants Malades INSERM U1151‐CNRS UMR 8253 Paris France

119. Department of Medicine IMedical Center ‐ University of FreiburgFaculty of MedicineUniversity of Freiburg Freiburg Germany

120. Universitaetsklinikum FreiburgLighthouse Core FacilityZentrum für Translationale Zellforschung, Klinik für Innere Medizin I Freiburg Germany

121. Immunology and EnvironmentLIMES InstituteUniversity of Bonn Bonn Germany

122. Owl Biomedical Inc. Santa Barbara USA

123. John van Geest Cancer Research CentreNottingham Trent University Nottingham UK

124. Department of MedicineAlbert Einstein College of Medicine Bronx NY USA

125. Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin, and Berlin Institute of HealthDepartment of Dermatology, Venereology and Allergology

126. Nanobiophotonics DepartmentLeibniz Institute of Photonic Technology (IPHT) Jena Germany

127. School of Plant Sciences and Bio5 InstituteUniversity of Arizona Tucson USA

128. Honorary Dean of Life SciencesHenan University Kaifeng China

129. Division of Molecular Oncology and Immunologythe Netherlands Cancer Institute Amsterdam The Netherlands

130. Institute of Experimental ImmunologyUniversity of Bonn Germany

131. Stanford Department of AnesthesiologyPerioperative and Pain MedicineStanford University School of Medicine CA USA

132. Fundação Oswaldo Cruz ‐ MinasLaboratory of Immunopatology Belo Horizonte MG Brazil

133. Department of MecicineUniversity of Massachusetts Medical School Worcester MA USA

134. INGM ‐ Fondazione Istituto Nazionale di Genetica Molecolare “Ronmeo ed Enrica Invernizzi” Milan Italy

135. Institute of ImmunologyDepartment of PathobiologyUniversity of Veterinary Medicine Vienna Austria

136. Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in PigsInstitute of ImmunologyDepartment of PathobiologyUniversity of Veterinary Medicine Vienna Austria

137. Department of DermatologyVenereology and Allergology, Charité ‐ Universitätsmedizin Berlin Berlin Germany

138. Translational Immunology InstituteSingHealth Duke‐NUS Academic Medical Centre Singapore

139. Shanghai Institute of ImmunologyDepartment of Immunology and MicrobiologyShanghai Jiao Tong University School of Medicine Shanghai China

140. Department of BioengineeringUniversity of California Los Angeles California USA

141. Department of ChemistryUniversity of Tokyo Tokyo Japan

142. Institute of Technological SciencesWuhan University Wuhan China

143. Institut für GenetikUniversität zu Köln Köln Germany

144. Alicante Institute for Health and Biomedical Research (ISABIAL) Alicante Spain

145. Networked Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd) Madrid Spain

146. Institute of Infection Immunity and InflammationCollege of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre Glasgow UK

147. Fondazione IRCCS Ca’ Granda, Ospedale Maggiore PoliclinicoUniversity of Milan

148. Cancer Immunology Research, Genentech South San Francisco CA USA

149. Friedrich‐Alexander‐University Erlangen‐Nürnberg (FAU)Department of Medicine 3Rheumatology and ImmunologyUniversitätsklinikum Erlangen Erlangen

150. Department of Internal Medicine and PediatricsFaculty of Medicine and Health Sciences Zwijnaarde Belgium

151. German Cancer Research Center (DKFZ) Heidelberg Germany

152. German Cancer Consortium (DKTK), partner site Berlin Berlin Germany

153. Department of Hematology, Oncology, and Tumor ImmunologyCharité – Universitätsmedizin Berlin, Campus Virchow Klinikum Berlin Germany

154. Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital Solna Sweden

155. Murdoch Children's Research Institute Parkville Victoria Australia

156. Charité – Universitätsmedizin Berlincorporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin

157. Department of Rheumatology and Clinical ImmunologyBerlin Institute of Health Berlin Germany

158. Flow CytometryHouston Methodist Hospital Research Institute Houston TX USA

159. Charité ‐ Universitätsmedizin BerlinMedical Department IDivision of GastroenterologyInfectiology and Rheumatology Berlin Germany

160. Cytometry ServiceIncliva Foundation. Clinic Hospital and Faculty of MedicineUniversity of Valencia Valencia Spain

161. Immunobiology Laboratory, Department of BiomedicineUniversity and University Hospital Basel Basel Switzerland

162. Cambridge Institute of Therapeutic Immunology & Infectious DiseaseJeffrey Cheah Biomedical CentreUniversity of Cambridge Cambridge UK

163. German Cancer Research Center (DKFZ)Division of Theoretical Systems Biology Heidelberg Germany

164. Center for ImmunologyUniversity of Minnesota Minneapolis MN USA

165. Leiden Computational Biology CenterLeiden University Medical Center Leiden The Netherlands

166. Computer Graphics and VisualizationDepartment of Intelligent SystemsTU Delft Delft The Netherlands

167. Karolinska Institutet Biomedicum Solna Sweden

168. Department of Experimental ImmunologyAmsterdam Infection and Immunity InstituteAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands

169. Department of HematopoiesisSanquin Research and Landsteiner LaboratoryAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands

170. Department of Chemical & Materials EngineeringNew Mexico State University Las Cruces NM USA

171. Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum EntzündungsmedizinUniversitätsklinikum Schleswig‐Holstein Kiel Germany

172. Department of Immunology & National Key Laboratory of Medical Molecular BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences (CAMS) & Peking Union Medical College Beijing China

173. Institute for Advanced Study (IAS)Shenzhen University Shenzhen China

174. Experimental ImmunologyHelmholtz Centre for Infection Research Braunschweig Germany

175. Department of HematologyOncology and RheumatologyUniversity Heidelberg Heidelberg Germany

176. Department of PathobiologySchool of Veterinary Medicine, University of Pennsylvania Philadelphia PA USA

177. Department of HematologySingapore General Hospital Singapore

178. Executive OfficesNational Cancer Centre Singapore Singapore

179. Department of Diagnostic MedicineClinical and Public Health, Univ. of Modena and Reggio Emilia Modena Italy

180. Max Planck Institute for Infection Biology Berlin Germany

181. Laboratory of CytomicsJoint Research Unit CIPF‐UVEGDepartment of Biochemistry and Molecular BiologyUniversity of Valencia Valencia Spain

182. Department of Histology and Embryology/Center for ProteomicsFaculty of Medicine, University of Rijeka Rijeka Croatia

183. Department of Paediatric Haematology and OncologySecond Faculty of MedicineCharles University Prague Czech Republic

184. Jena University HospitalInstitute of Immunology Jena Germany

185. Department of Rheumatology and Clinical ImmunologyMedical Center ‐ University of FreiburgFaculty of Medicine, University of Freiburg Freiburg Germany

186. Center for Chronic ImmunodeficiencyMedical Center ‐ University of FreiburgFaculty of MedicineUniversity of Freiburg Freiburg Germany

187. Department of Microbiology and ImmunologyYong Loo Lin School of MedicineNational University of Singapore Singapore

188. Institute of Molecular Health SciencesETH Zurich Zürich Switzerland

189. Department of NeuropathologyUniversitätsklinikum Erlangen Germany

190. John W Deming Endowed Chair in Internal MedicineCenter for Translational Research in Infection and Inflammation Tulane School of Medicine New Orleans LA USA

191. Department of NeurologyTechnical University of Munich Munich Germany

192. Department of RheumatologyLeiden University Medical Center Leiden The Netherlands

193. Division of Cell BiologyHistology & EmbryologyGottfried Schatz Research CenterMedical University of Graz Graz Austria

194. Institute for Molecular MedicineGoethe University Frankfurt Frankfurt am Main Germany

195. FACS & Imaging Core FacilityMax Planck Institute for Biology of Ageing Cologne Germany

196. Flow & Mass Cytometry Core FacilityCharité ‐ Universitätsmedizin Berlin and Berlin Institute of Health Berlin Germany

197. BCRT Flow Cytometry LabBerlin‐Brandenburg Center for Regenerative TherapiesCharité ‐ Universitätsmedizin Berlin

198. WPI Immunology Frontier Research CenterOsaka University Osaka Japan

199. School of Biological SciencesNanyang Technological University Singapore

200. INSERM U932PSL UniversityInstitut Curie Paris France

201. Department of Medicine and Aging SciencesCentre on Aging Sciences and Translational Medicine (Ce.S.I.‐Me.T.), University “G. d'Annunzio” of Chieti‐Pescara Chieti Italy

202. Institut Cochin, CNRS8104, INSERM1016Department of Endocrinology, Metabolism and DiabetesUniversité de Paris Paris France

203. Section of ImmunologyVetsuisse FacultyUniversity of Zurich Zurich Switzerland

204. The Human Immune Monitoring Center (HIMC)Institute for ImmunityTransplantation and InfectionStanford University School of Medicine CA USA

205. School of Biomedical EngineeringThe University of British Columbia Vancouver Canada

206. Caprion BiosciencesImmuneCarta Montréal Quebec Canada

207. Center for Infectious MedicineDepartment of Medicine HuddingeANA Futura, Karolinska Institutet Stockholm Sweden

208. Inst. f. Med. Mikrobiology and Hospital HygieneUniversity of Marburg Germany

209. King's College London“Peter Gorer” Department of Immunobiology London UK

210. Beckman Coulter, Inc Miami FL USA

211. IMIM(Hospital de Mar Medical Research Institute)University Pompeu Fabra Barcelona Spain

212. Department of Microbial Infection and ImmunityOhio State University Columbus OH USA

213. Department of RheumatologyErasmus MC, University Medical Center Rotterdam Rotterdam The Netherlands

214. Centre d'Immunophénomique ‐ CIPHE (PHENOMIN)Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367) Marseille France

215. Institute of ImmunobiologyKantonsspital St.Gallen St. Gallen Switzerland

216. Department of Virus ImmunologyHeinrich Pette InstituteLeibniz Institute for Experimental Virology Hamburg Germany

217. Institute for ImmunityTransplantation, and Infection, Stanford University School of Medicine Stanford CA USA

218. Flow and Image Cytometry Shared ResourceRoswell Park Comprehensive Cancer Center Buffalo NY USA

219. Fred Hutchinson Cancer Research CenterVaccine and Infectious Disease Division Seattle WA USA

220. Istituto Clinico Humanitas IRCCS and Humanitas University Pieve Emanuele Milan Italy

221. William Harvey Research InstituteQueen Mary University London United Kingdom

222. Department of ImmunologyRady Faculty of Health Sciences, University of Manitoba Winnipeg MB Canada

223. Cytomics Technological ServicePrincipe Felipe Research Center Valencia Spain

224. National Institute of GeriatricsRheumatology and RehabilitationDepartment of Pathophysiology and Immunology Warsaw Poland

225. Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie MedicheUniversità di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS‐CNR) Napoli Italy

226. Lab of Clinical and Experimental ImmunologyHumanitas Clinical and Research Center Rozzano Milan Italy

227. Cell Clearance in Health and Disease LabVIB Center for Inflammation Research Ghent Belgium

228. Department of Biomedical Molecular BiologyGhent University Ghent Belgium

229. Ramaciotti Facility for Human Systems Biology, and Discipline of PathologyThe University of Sydney Camperdown Australia

230. Clinical Trial Center LeipzigUniversity Leipzig Leipzig Germany

231. Interdepartmental Immunobiology CenterDept. of Microbiology‐ImmunologyNorthwestern Univ. Medical School Chicago IL USA

232. Trinity College Dublin, School of Biochemistry and ImmunologyTrinity Biomedical Sciences Institute Dublin Ireland

233. Department of Clinical and Experimental MedineLinköping University Linköping Sweden

234. Abramson Cancer Center Flow Cytometry and Cell Sorting Shared ResourcePerelman School of Medicine at the University of Pennsylvania Philadelphia PA USA

235. Department of ImmunologyIRCCS Bambino Gesu Children's Hospital Rome Italy

236. David H. Smith Center for Vaccine Biology and ImmunologyUniversity of Rochester Medical Center Rochester NY USA

237. Centre for Environmental Research ‐ UFZDepartment Environmental Microbiology Leipzig Germany

238. Institute for Innovative Radiotherapy (iRT)Experimental Immune Biology, Helmholtz Zentrum München Neuherberg Germany

239. Radiation Immuno‐Oncology GroupCenter for Translational Cancer Research Technische Universität München (TranslaTUM)Klinikum rechts der Isar Munich Germany

240. Department of ImmunologyGraduate School of MedicineChiba University Chiba city Chiba Japan

241. Institute of Experimental Immunology and HepatologyUniversity Medical Center Hamburg‐Eppendorf Hamburg Germany

242. Discipline of DermatologyUniversity of Sydney Sydney New South Wales Australia

243. State Key Laboratory of Experimental HematologyInstitute of HematologyChinese Academy of Medical Sciences & Peking Union Medical College Tianjin China

244. Barts and The London School of Medicine and DentistryQueen Mary University of London UK

245. Department of Pathology and Rogel Cancer Centerthe University of Michigan Ann Arbor Michigan USA

246. Flow Cytometry Core FacilityEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany

247. Department of MedicineCancer Research Centre (IBMCC‐CSIC/USAL)Cytometry ServiceUniversity of SalamancaCIBERONC and Institute for Biomedical Research of Salamanca (IBSAL) Salamanca Spain

248. Burnet InstituteAMREP Flow Cytometry Core Facility Melbourne Victoria Australia

249. Inflammation and OncologyResearch, Amgen Inc South San Francisco USA

250. ETH ZurichInstitute of Microbiology Zurich Switzerland

251. Department of Transfusion MedicineInstitute of Immunology and Transfusion MedicineUniversity Medicine Greifswald Greifswald Germany

252. Center of Excellence for Flow CytometryDepartment of Research and DevelopmentFaculty of Medicine Siriraj Hospital, Mahidol University Bangkok Thailand

253. Institute of Biomedicine and Translational MedicineUniversity of Tartu Tartu Estonia

254. Functional Cytomics GroupJosep Carreras Leukaemia Research InstituteCampus ICO‐Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB Badalona Spain

255. Institute of ImmunologyCenter for PathophysiologyInfectiology and Immunology, Medical University of Vienna Vienna Austria

256. Department of Life SciencesUniversity of Modena and Reggio Emilia Modena Italy

257. Chromocyte LimitedElectric Works Sheffield UK

258. Department for Internal Medicine 3Institute for Rheumatology and ImmunologyAG Munoz, Universitätsklinikum Erlangen Erlangen Germany

259. Department of ImmunologyUniversity of Udine Udine Italy

260. School of Biomedical and Health SciencesRMIT University Bundoora Victoria Australia

261. Department of Biochemistry and Molecular BiologyMonash University Clayton Victoria Australia

262. Department of Rheumatology and Clinical ImmunologyUniversity Medical Center UtrechtUtrecht University Utrecht The Netherlands

263. Regeneration in HematopoiesisLeibniz‐Institute on AgingFritz‐Lipmann‐Institute (FLI) Jena Germany

264. Preparative Flow Cytometry, Max‐Delbrück‐Centrum für Molekulare Medizin Berlin Germany

265. Bindley Biosciences CenterPurdue University West Lafayette IN USA

266. Department of Internal MedicineGroene Hart Hospital Gouda The Netherlands

267. Desatoya LLC Reno NV USA

268. Immunobiology LaboratoryThe Francis Crick Institute London UK

269. Renal Transplant UnitDivision of Internal MedicineAcademic Medical CentreAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands

270. Core Facility Flow CytometryBiomedical Center, Ludwig‐Maximilians‐University Munich Germany

271. Department of Medical Microbiology and ImmunologyUniversity of Alberta Alberta Canada

272. Purdue University Cytometry LaboratoriesPurdue University West Lafayette IN USA

273. Cell Biology UnitIRCCS Ospedale Policlinico San Martino Genova Italy

274. Institut für Klinische Chemie und PathobiochemieFakultät für MedizinTechnische Universität München München Germany

275. Data Mining and Modeling for BiomedicineVIB‐UGent Center for Inflammation Research Ghent Belgium

276. Department of Applied Mathematics, Computer Science and StatisticsGhent University Ghent Belgium

277. RIKEN Center for Integrative Medical Sciences Yokohama Japan

278. Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and BiologyUniversity of Lausanne Epalinges Switzerland

279. Heidelberg UniversityInstitute of ImmunologySection of Molecular Immunology Heidelberg Germany

280. Translational Immunology LaboratoryNIHR BRCUniversity of OxfordKennedy Institute of Rheumatology Oxford UK

281. Department of Molecular MedicineSapienza University of RomeIRCCS Neuromed Pozzilli Italy

282. Centre de Recherches des CordeliersEquipe Cancer et Immunité anti‐tumorale Paris France

283. Charité – Universitätsmedizin Berlin, and Berlin Institute of HealthInstitute of Medical Immunology Berlin Germany

284. Clinic for Orthopedics and Trauma SurgeryUniversity Hospital Bonn Bonn Germany

285. R&D ReagentsMiltenyi Biotec GmbH Bergisch Gladbach Germany

286. Quantitative Systems BiologyLife & Medical Sciences InstituteUniversity of Bonn Bonn Germany

287. Internal Medicine I, University Hospital Regensburg Germany

288. Flow Cytometry Core FacilityGerman Cancer Research Centre (DKFZ) Heidelberg Germany

289. Genome Biology UnitEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany

290. Institute of Molecular and Clinical ImmunologyOtto‐von‐Guericke University Magdeburg Germany

291. University of CambridgeCambridge Institute for Medical Research Cambridge UK

292. Institut PasteurCellular Lymphocytes BiologyImmunology Departement Paris France

293. TRI Flow Cytometry Suite (TRI.fcs)Translational Research Institute Wooloongabba QLD Australia

294. AsedaSciences West Lafayette IN USA

295. Instituto de Medicina Molecular João Lobo AntunesFaculdade de MedicinaUniversidade de Lisboa Portugal

296. Dept. Molecular Translational MedicineUniversity of Brescia Brescia Italy

297. Department of OncologyUniversity of Lausanne and CHUV Epalinges Switzerland

298. BD Life Sciences Ashland OR USA

299. Lundberg Laboratory for CancerDepartment of PathologySahlgrenska Academy at University of Gothenburg Gothenburg Sweden

300. BD Life Sciences San Diego CA USA

301. Institute for Hygiene and Applied ImmunologyCenter for PathophysiologyInfectiology and ImmunologyMedical University of Vienna Vienna Austria

302. National Institutes of Health Bethesda MD USA

303. Departement for Therapy ValidationFraunhofer Institute for Cell Therapy and Immunology IZI Leipzig Germany

304. Institute for Medical InformaticsStatistics and Epidemiology (IMISE)University of Leipzig Leipzig Germany

305. Department of Precision InstrumentsTsinghua University Beijing China

306. BioGenes GmbH Berlin Germany

307. Novartis Biologics CenterMechanistic Immunology UnitNovartis Institute for Biomedical ResearchNIBR Basel Switzerland

308. Department of Cellular and Molecular PhysiologyPenn State University College of Medicine PA United States

309. Institute of BioinnovationBSRC Alexander Fleming Vari Greece

310. Department of Biochemistry, Institute of ChemistryUniversity of São Paulo São Paulo SP Brazil

311. Christine Kühne Center for Allergy Research and Education (CK‐CARE) Davos Switzerland

312. Vesicle Observation CenterBiomedical Engineering & PhysicsLaboratory Experimental Clinical ChemistryAmsterdam University Medical Centers Location AMC The Netherlands

313. VIB Flow CoreVIB Center for Inflammation Research Ghent Belgium

314. Department of Infection BiologyUniversity Hospital ErlangenFriedrich‐Alexander University Erlangen‐Nuremberg (FAU) Erlangen Germany

315. BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of HealthCore Unit ImmunoCheck

316. A·P·E‐Applied Physics & Electronics Berlin Germany

317. Institute for Molecular MedicineUniversity Medical Center of the Johannes Gutenberg University of Mainz Mainz Germany

318. Flow Cytometry FacilityBabraham Institute Cambridge UK

319. Roswell Park Comprehensive Cancer CenterElm and Carlton Streets Buffalo NY USA

320. Dept of HematopathologyThe University of Texas MD Anderson Cancer Center Houston TX USA

321. The Scientific Platformsthe Westmead Institute for Medical Researchthe Westmead Research Hub Westmead New South Wales Australia

322. Flow Cytometry Core FacilityBlizard InstituteQueen Mary London University London UK

323. Faculty of Biological SciencesFriedrich Schiller University Jena Jena Germany

324. Medinfomatics Ltd. West Drayton Middlesex UK

325. Department for ImmunologyLeibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo) Dortmund Germany

326. Institute for Cellular & Molecular ImmunologyUniversity Medical Center Göttingen Göttingen Germany

327. Department of Infectious DiseaseImperial College London UK

328. Wellcome Centre for Infectious Diseases Research in Africa and Department of MedicineInstitute of Infectious Disease and Molecular MedicineUniversity of Cape Town, Republic of South Africa

329. Tuberculosis LaboratoryThe Francis Crick Institute London UK

330. Cooperation Unit for Experimental and Translational Cancer ImmunologyInstitute of Immunology (Charité ‐ Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ) Berlin Germany

331. University BonnMedical Faculty Bonn Germany

332. BD Biosciences San Jose CA USA

333. School of Computing ScienceSimon Fraser University Burnaby Canada

334. Department of Rheumatology and ImmunologyWest China Hospital, Sichuan University Chengdu Sichuan China

335. Institute of VirologyTechnical University of Munich Munich Germany

336. TranslaTUMTechnical University of Munich Munich Germany

337. Maurice Müller Laboratories (Department of Biomedical Research)Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern Bern Switzerland

Publisher

Wiley

Subject

Immunology,Immunology and Allergy

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