An efficient scheme for purification of a novel recombinant immunotoxin, rCCK8PE38, for anti-tumour experiments

Author:

Hu Pan1ORCID,Zhang Song1ORCID,Lu Shi-ying1,Li Meng1,Chang Jiang1,Wang Meng-yun1,Li Chang1,Zhao Ke1,Guan Yu-Ting1,Zhang Yuan-Yuan1,Li Yan-Song1,Zhou Yu1,Liu Zeng-Shan1,Bai Ou2,Ren Hong-Lin1

Affiliation:

1. Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine; Jilin University; Changchun China

2. Department of Oncology; First Hospital of Jilin University; Changchun China

Funder

National Natural Science Foundation Young Investigator Grant Program

Youth Research Fund of Science and Technology Development Plan of Jilin Province

Class General Financial and special Financial Grant from the China Postdoctoral Science Foundation

Key Project in Jilin Province

Specialized Research Fund for Doctoral Program of Colleges and Universities

Publisher

Wiley

Subject

Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry

Reference27 articles.

1. Immunotoxin: A new tool for cancer therapy;Allahyari;Tumour Biology,2017

2. Stress-free chromatography: IEC and HIC;Arakawa;Current Pharmaceutical Biotechnology,2009

3. An improved recombinant Fab-immunotoxin targeting CD22 expressing malignancies;Bera;Leukemia Research,2014

4. Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site;Chant;Protein Expression and Purification,2005

5. Refolding and purification of recombinant immunotoxin denileukin diftitox;Ebrahimi;Iranian Journal of Public Health,2016

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