High cell density transient transfection of CHO cells for TGF‐β1 expression
Author:
Affiliation:
1. Institute of Technical Chemistry Gottfried Wilhelm Leibniz University of Hannover Hannover Germany
2. Chemistry of Natural and Microbial Products Department Pharmaceutical and Drug Industries Research Division National Research Centre Giza Egypt
Funder
Deutscher Akademischer Austausch Dienst Kairo
Publisher
Wiley
Subject
Bioengineering,Environmental Engineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/elsc.201800174
Reference44 articles.
1. Automation of large scale transient protein expression in mammalian cells
2. Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency
3. Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale
4. Cell line specific control of polyethylenimine-mediated transient transfection optimized with “Design of experiments” methodology
5. Extended gene expression by medium exchange and repeated transient transfection for recombinant protein production enhancement
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