Postoperative immunological function and jaundice

Abstract

Abstract The effects of operative trauma and obstructive jaundice on systemic immunity were studied in a rat model, using the delayed-type hypersensitivity response to 2,4-dinitro-1-fluorobenzene as a measure of systemic immune responsiveness. Midline laparotomy caused a significant decrease in the mean(s.e.m.) delayed-type hypersensitivity response 1 week after operation (4·6(1·3) versus 19·0(2·2) per cent for controls). The response returned to control leyels by 2 weeks (14·6(3·1) per cent). Common bile duct ligation and division resulted in a significantly depressed hypersensitivity response at 2 and 3 weeks (6·8(2·0) and 8·4(1·6) per cent respectively). The expected decrease in the response at 1 week in these animals was not observed (mean(s.e.m.) 12·7(2·7) per cent), suggesting a possible role for the normal liver in the induction of postoperative immune depression. Impaired function of the reticuloendothelial system was induced in non-jaundiced animals by Kupffer cell ablation following intraportal infusion of λ-carrageenan. A similar prevention of postoperative immune hyporesponsiveness was observed (mean(s.e.m.) 10·4(1·0) versus 10·4(1·6) per cent for controls). Hepatic Kupffer cells play an important role in the induction of postoperative immune depression.