Experimental organ preservation

Abstract

Abstract An experimental model for the study of organ preservation based on the isolated, biologically perfused rat heart is described. Using this model a standard storage solution has been evolved for use with initial perfusion and hypothermic storage. This solution contains Dextran 40 and insulin. Various perfusion characteristics including pH and infusion volume have also been studied. This solution has been modified and compared with two currently available storage solutions–Collins's C4 solution and the Gelin technique–and found to be superior to both. The technique consists of two parts; first, the organ is flushed with 0.5 per cent lignocaine in a buffered solution and is then perfused with the Dextran-40-based solution containing insulin and lignocaine. Further modification of the solution to contain a high magnesium concentration has resulted in a further improvement in survival after storage. These results are now being confirmed in the dog kidney.