Electrochemical Investigation of Viral Respiratory Infection Inflammatory Biomarker Serum Amyloid A Protein by Using PtNP Modified Electrode

Author:

Kalpana 1,Ghrera Aditya Sharma1ORCID

Affiliation:

1. The NorthCap University Applied Science Department Gurugram Haryana India 122017

Abstract

AbstractThe present study reports the optimisation of electrochemical deposition parameters for platinum nanoparticles (PtNPs) on (Indium‐tin‐oxide) surface. PtNPs were electrochemically deposited on APTES (3‐Aminopropyl) triethoxysilane) modified ITO surface to prepare working electrode (PtNP/APTES/ITO). This electrode is further used to fabricate immunoelectrode for detection of SAA biomarker (APO‐SAA) by SAA specific antibodies (SAA Ab) which are immobilised by SAM on the surface of PtNP/APTES/ITO to fabricate SAA‐Ab/PtNP/APTES/ITO immunoelectrode. Characterization of prepared immunoelectrode is done by electrochemical characterization techniques like Differential Pulse Voltammetry (DPV), Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Scanning Electron Microscopy (SEM). Characterization by electrochemical techniques reveal that SAA‐Ab/PtNP/APTES/ITO immunoelectrode can detect Serum Amyloid A protein (SAA) when 10 μl of it (from initial concentration of 10 fg/ml to 106 fg/ml) is poured on immunoelectrode and kept for 10 minutes. Linearity equation is obtained and the LOD is found to be 8.22 fg/ml by applying equation 3σ/sensitivity.

Publisher

Wiley

Subject

General Chemistry

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3