Preparation and Characterization of Alkali Activated Carbon Based Theobroma Cocoa Pods: Application for Electrochemical Determination of Xanthine in Fresh Fish Sample

Author:

Somba Alex Vincent1,Kamgaing Théophile1,Fotsop Cyrille Ghislain2,Deffo Gullit1ORCID,Nkuigoua Bibiane Wandji1,Tagne Rufis Fregue Tiegam3,Tajeu Kevin Yemele1,Temgoua Ranil Clément Tonleu1,Njanja Evangeline1,Tonlé Ignas Kenfack1

Affiliation:

1. Electrochemistry and Chemistry of Materials Department of Chemistry Faculty of Science University of Dschang P O Box 67 Dschang Cameroon

2. Institute of Chemistry Faculty of Process and Systems Engineering Universität Platz 2 39106 Magdeburg Germany

3. Department of Paper Sciences and Bioenergy University Institute of Wood Technology University of Yaoundé I Mbalmayo

Abstract

AbstractIn order to propose an alternative for the sensors made with complex materials, a cheap and facile electrode material based on the preparation of activated carbon from cocoa pods (Theobroma cacao) has been proposed for the electroanalysis of xanthine (Xa). Raw cocoa pods (RCP) and activated carbon cocoa pods (ACCP) were prepared and characterized by pH at the zero point of charge, FTIR, SEM/SEM‐mappings, EDX, XRD, Raman spectroscopy, BET/BJH analysis, 13C NMR, and TGA/DSC. RCP and ACCP were drop coated on the surface of a glassy carbon electrode (GCE) and characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The electrodes GCE/RCP and GCE/ACCP were used through differential pulse voltammetry to optimize the different parameters that can affect the determination of Xa and a calibration curve has been plotted in the range from 1.0 to 12.0 μM, leading to a detection limit of 0.264 μM. The interference, reproducibility, and stability were evaluated and the sensor was successfully applied in fresh fish sample. Therefore, the simple way of preparation of ACCP and the significant role played by GCE/ACCP in resolving the oxidation of Xa is expected to impact significantly the future sensor research through substitution of the costly and hazardous materials.

Funder

German Academic Exchange Service

Publisher

Wiley

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