A Novel Coumarin‐Based Fluorescent Probe for Sequential Detection of Al3+ and H2PO4−

Abstract

AbstractA coumarin‐derived acylhydrazone probe (L) was designed and synthesized for successive detection of Al3+ and H2PO4− in DMSO/H2O solution. The probe L exhibited high selectivity towards Al3+ with visible color change and prominent fluorescence enhancement at 592 nm, while the in situ prepared L‐Al3+ complex could selectively recognize of H2PO4− via fluorescence quenching. The detection limits for Al3+ and H2PO4− were calculated to be 3.4 and 11.0 nM, respectively. The binding ratio of L to Al3+ was obtained as 2 : 1 according to the Job's plot and ESI‐MS while the association constant was determined to be 2.13×104 M−1. Furthermore, the probe L was successfully applied in detection of Al3+ and H2PO4− with test strips and in real water samples.