Dicyanostilbene‐Derived Highly Sensitive Two‐Photon Fluorescence Temperature Probe for Live Cell Imaging

Author:

Huang Chibao12ORCID,Wang Qian1,Yan Xiong1

Affiliation:

1. School of Information Engineering Zunyi Normal University Zunyi 563002 China

2. State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China

Abstract

AbstractA novel two‐photon fluorescence temperature‐sensitive probe (SP) with the push‐pull electronic architecture (Donor‐π‐Acceptor, D‐π‐A) derived from dicyanostilbene was developed. Its linearly dependent coefficients (R2) between the emission intensity of SP and temperature reached 0.998 and 0.999 in one‐ and two‐photon fluorescence (OPF and TPF), respectively, and corresponding function expressions were IF=−0.0144T+1.2751 (OPF) and IF=−0.0152T+1.2970 (TPF). Its maximum two‐photon excitation wavelength (λmax2) is 750 nm, and the corresponding maximum two‐photon absorption cross‐section (δmax) is 1550 GM. DFT (Density Functional Theory) Calculation demonstrated that the electron cloud densities of two nitrogen atoms (N29 and N31) on two cyano groups are 15.9 and 16.0 times higher than that of nitrogen atom on 9‐carbazolyl group (N13), respectively. This fully indicates that the electron cloud of nitrogen atom on 9‐carbazolyl group is highly delocalized to the fluorophore matrix. SP can be used for live cell imaging and detect cell temperature to diagnose disease within physiological temperature range with excellent photostability and without cytotoxicity.

Publisher

Wiley

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