Fabrication of Carbon Paste Sensor Activated with Electropolymerized DL‐Phenylalanine for the analysis of Levofloxacin

Abstract

AbstractVoltammetric analysis of Levofloxacin (LF) using an electrochemically polymerized composite of DL‐phenylalanine (DLP) alongside a bare carbon paste electrode. The constructed electrodes are polymerized DL‐phenylalanine modified carbon paste electrodes P(DLP/MCPE) and bare carbon paste electrodes (BCPE). Cyclic voltammetry (CV), differential pulse voltammetry (DPV), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and other electrochemical techniques used for characterization of P(DLP/MCPE) and BCPE in 0.2 M phosphate buffer solution (PBS) with pH 4.5 were conformed using the P(DLP/MCPE), and it shows the highest current responses compared to the BCPE. This research work examines the effect of pH in PBS, scan rate, and LF concentration on the surface of P(DLP/MCPE). The outcome result shows that the oxidation reaction of LF pH is an adsorption‐controlled path. The detection limit of LF ranges from 0.2 μM to 7.0 μM, and the lower limits of detection (LOD) is 0.613x10−7 M and the lower limits of quantification (LOQ) is 2.051X10−7 M. The electrochemical P(DLP/MCPE) shows good repeatability, reproducibility, and stability. Finally, by analyzing LF in a pharmacological sample, the real time application of the sensor was assessed, and significant recoveries were observed.