Preparation and Characterization of Biowaste Derived Zn, Sr Co‐substituted HAP/Lignin Biocomposite for Potential Biomedical Applications

Abstract

AbstractThis present study deals with the facile synthesis of Zinc (Zn), Strontium (Sr) Co‐substituted hydroxyapatite (M‐HAP)/lignin composite for biomedical applications. For composite formation, HAP was isolated from egg shell through wet precipitation method, biopolymer of lignin was extracted from neem wood through organosolve technique then HAP composites (HAP, HAP/lignin, Zn‐HAP/lignin, Sr‐HAP/lignin, Zn, Sr‐HAP/lignin) were obtained using freeze drying performance. The synthesized HAP composites were characterized using X‐ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Energy‐dispersive X‐ray analysis (EDAX). The antibacterial behavior of the HAP composites was investigated against E. Coli, B. Subtilies, P. Aeruginosa and S. Aureus pathogens. Then the mechanical property of the HAP composites was carried out using Vicker's microhardness test which reveals a better improvement in mechanical hardness. The antibacterial activity confirms that the HAP composite exhibits enhanced antibacterial activity. Further, the cell viability analysis shows that the co‐substitution of Zn and Sr in HAP lattice improved cell viability analysis, which indicates that the obtained Zn, Sr co‐substituted HAP/lignin biocomposite acts as a potential biomaterial for better biomedical application.