Determination of Antioxidant Activities of Viscum album L.: First Report on Interaction of Phenolics with Survivin Protein using in silico Analysis

Abstract

AbstractMistletoe, Viscum album L., (VA) known as “Ökse Otu” in Turkey, is a hemiparasitic shrub that grows especially on the trunks and crowns of broadleaf trees. This study is aimed to determination of the VA’s antioxidant compounds and evaluate their in vitro activities growing on twelve different host trees and determination of in silico approach of compounds against survivin protein. At the same time, the active ingredients determined in HPLC analysis, were performed with molecular docking and molecular dynamics analysis on survivin protein, an apoptosis inhibitor protein in cancer. Antioxidant activities of methanolic extracts of organs of VA were determined. Phytochemical properties of different organs of VA samples taken from different hosts varied as well. Total phenolic content was determined at the highest rate in fruits, followed by leaves and branches, respectively. Total flavonoid content was determined in the highest amounts in the leaves, followed by branches and fruits, respectively. Antioxidant activity values were evaluated with DPPH (using DPPH as a radical), ABTS (using ABTS as radical cation) and FRP tests. The antioxidant activity of the branches of the plant was determined at the highest rate, followed by leaves and fruits, respectively. During in silico analysis, Quercetin, Rosmarinic acid and Catechin were found to bind to survivin protein effectively. These substances can have the potential to become active pharmaceutical ingredients by working with different proteins in the future.