Affiliation:
1. Department of Stomatology Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science Xiangyang 441000 PR China
2. Department of Stomatology The First Affiliated Hospital Fujian Medical University Fuzhou 350005 PR China
3. Department of Stomatology Xiangyang No.1 People's Hospital Hubei University of Medicine Xiangyang 441053 PR China
Abstract
AbstractDesigning sensitive and low‐cost method for interleukin‐8 (IL‐8) determination is of great significance in the early diagnosis of oral cancer. In the present work, a simple, low‐cost and sensitive sandwich‐like electrochemical immunosensor of IL‐8 was developed by introducing perylene tetracarboxylic acid‐carbon black (PTCA‐CB) as sensing platform and silver‐ferrocene (Ag−Fc) as signal amplifier. PTCA‐CB can offer large surface area and high conductivity as well as the stable supporter to assemble primary antibody (Ab1), while Ag could carry Fc probe and secondary antibodies (Ab2) to produce Ab2−Ag−Fc signal amplifier. In the presence of IL‐8 with different concentrations, the peak currents from the formed sandwich‐like structure Ab2−Ag−Fc/IL‐8/Ab1/CB can increase with the increase of IL‐8 concentration, a sensitive and low‐cost electrochemical immunosensor for IL‐8 was thus constructed based on this principle. After optimizing the related control conditions, a wide linear range (3.0 pg mL−1 to 2.0 ng mL−1) and very low detection limit (1.0 pg mL−1) were achieved, it's thus convinced the developed sensor has important clinical applications.
Funder
Fujian Provincial Health and Family Planning Commission
Cited by
2 articles.
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