Affiliation:
1. Faculty of Pharmaceutical Science Tohoku Medical and Pharmaceutical University Sendai Japan
2. Department of Creative Engineering National Institute of Technology, Tsuruoka College Tsuruoka Japan
3. School of Pharmaceutical Sciences Ohu University Koriyama Japan
4. Department of Electronics Graduate School of Engineering Nagoya University Nagoya Japan
5. Nano Life Science Institute (WPI‐NanoLSI) Kanazawa University Kanazawa Japan
Abstract
AbstractIn this study, glucose oxidase (GOx) was immobilized on the electrode surface by layer‐by‐layer and gel membrane technique and characterized the GOx immobilized film morphology, H2O2 permeability, and glucose response. Concanavalin A (Con A)‐GOx multilayer electrodes showed higher glucose‐related current response than GOx‐bovine serum albumin gel membrane‐coated electrode, a common modification method. The thin thickness of the Con A/GOx multilayer film efficiently catalyzed the enzymatic reaction, and H2O2 was produced near the electrode surface, resulting in an immediate electrode response.
Funder
Japan Society for the Promotion of Science
Takahashi Industrial and Economic Research Foundation
Kurita Water and Environment Foundation
Mitani Foundation for Research and Development
Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering
Japan Science and Technology Corporation
Japan Agency for Medical Research and Development