Low-density subculture: a technical note on the importance of avoiding cell-to-cell contact during mesenchymal stromal cell expansion
Author:
Affiliation:
1. School of Materials, Faculty of Engineering and Physical Sciences; University of Manchester; UK
2. Centre for Tissue Injury and Repair, Institute of Inflammation and Repair, Faculty of Medical and Human Sciences; University of Manchester; UK
Funder
Arthritis Research UK and Mr Andrew Fotheringham
Publisher
Wiley
Subject
Biomedical Engineering,Biomaterials,Medicine (miscellaneous)
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1002/term.2051/fullpdf
Reference18 articles.
1. Mesenchymal stem cell therapy: two steps forward, one step back;Ankrum;Trends Mol Med,2010
2. Suitability of human mesenchymal stem cells for gene therapy depends on the expansion medium;Apel;Exp Cell Res,2009
3. Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: implications for their use in cell therapy;Banfi;Exp Hematol,2000
4. Two steps to functional mesenchymal stromal cells for clinical application;Bartmann;Transfusion,2007
5. Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms;Bernardo;Cancer Res,2007
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