Affiliation:
1. College of Chemistry State Key Laboratory of Elemento-Organic Chemistry Key Laboratory of Functional Polymer Materials (Ministry of Education) Frontiers Science Center for New Organic Matter Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 China
Abstract
AbstractDetection of phenylpyruvic acid (PPA) is important for the diagnosis of phenylketonuria (PKU). In this study, we designed a guanidinomethyl‐modified calix[5]arene that can strongly and selectively bind with PPA. Via indicator displacement assay, we set up a calibration line for accurately determining PPA concentration down to the μM range in artificial urine based on the linear relationship between the fluorescence intensity and PPA concentration. This work provides a low‐cost, easy‐to‐operate, rapid, and sensitive method for PPA detection, and may offer an alternative for PKU diagnosis in clinical study.
Funder
National Natural Science Foundation of China
China Postdoctoral Science Foundation