Affiliation:
1. Department of Chemistry University of Wisconsin-Madison 1101 University Ave Madison WI 53706 USA
2. School of Pharmacy University of Wisconsin-Madison 777 Highland Ave Madison WI 53705 USA
Abstract
AbstractLipidomic analysis of human serum is essential to monitor the individual's health status. Herein, we develop a facile strategy for rapid characterization of phospholipids in human serum via indium tin oxide (ITO) coated glass slide solid phase extraction MALDI mass spectrometry (ITO‐SPE‐MALDI‐MS). Phospholipid species are retained on ITO slide via solid phase extraction owing to the unique property of the ITO material; the measurement of phospholipid species from 1 μl human serum within 2 min is achievable. A comparison of ITO‐SPE strategy with conventional extraction methods was further carried out using liquid chromatography‐mass spectrometry (LC‐MS) and ion‐mobility mass spectrometry (IM‐MS), resulting in a comparable enrichment performance for the phospholipid analysis. Furthermore, rapid lipidomic profiling of serum samples from human colorectal cancer patients and cell lines was demonstrated. Our results indicate that ITO‐SPE‐MALDI‐MS provides a higher throughput strategy for the analysis of phospholipid species in complex biological mixtures, showcasing its potential for applications in the analysis of clinical biofluids.
Funder
National Institutes of Health
American Society for Mass Spectrometry
University of Wisconsin Carbone Cancer Center
Wisconsin Alumni Research Foundation